A survey on the distribution of viruses in wild Japanese flounder Paralichtys olivaceous and other marine fishes

野生牙鲆等海洋鱼类病毒分布调查

• 批准号: 12660172
• 负责人: NISHIZAWA Toyohiko
• 金额: $ 1.98万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660172/
• 关键词: Aquabirnavirus; VHSV; YTAV; Japanese flounder; wild fish; virus isolation; 天然ヒラメ; 魚類病原ウイルス; 病原性

For a survey on the distribution of fish pathogenic viruses among wild Japanese flounder Paralichthys olivaceus, 434 fish were collected in 14 coastal areas of Japan in 1999 and 2001. Using established fish cell lines, aquabirnaviruses were isolated from 135 out of 358 fish (38%) caught in almost all the coastal areas and the representative isolate was identified as YTAV (yellowtail ascites virus) by cross-neutralization tests. In addition to the aquabirnavirus, VHSV (viral hemorrhagic septicemia virus) was isolated from 34 out of 434 fish (8%) collected in Wakasa Bay, Minami Izu and the Seto Inland Sea mainly in winter and spring. In a pathogenicity test, 4 isolates of aquabirnaviruses did not cause any mortality or morbidity in young Japanese flounder by intramascular injection at 10^<3.0> - 10^<7.6> TCID_<50>/fish. On the other hand, mortalities of 60% and 100% were produced by the inoculation of an isolate of VHSV at 10^<2.0> and 10^<6.0> TCID_<50>/fish, respectively, in flounder. Virus isolation trials were also performed from wild Japanese horse mackeral Trachurus japonicus (141 fish), darkbanded rockfish Sebastes inermis (101 fish) and other marine fishes (134 fish with 7 fish species) in 2000 and 2001, but only aquabirnaviruses were isolated from some of them.

为了调查鱼类病原性病毒在野生日本比目鱼中的分布，于1999年和2001年在日本14个沿海地区采集了434条鱼。使用已建立的鱼类细胞系，从几乎所有沿海地区捕获的358条鱼中的135条（38%）中分离出水病毒，通过交叉中和试验将代表性分离物鉴定为YTAV（黄尾腹水病毒）。在若狭湾、南伊豆和濑户内海采集的434条鱼中，除水病毒RNA病毒外，还分离到34条（8%）VHSV（病毒性出血性败血症病毒），主要在冬季和春季。在致病性试验中，4株水病毒分离物以10^ - 10^ TCID_ /鱼的剂量肌内注射未引起幼比目鱼的死亡或发病<3.0><7.6><50>。另一方面，将VHSV分离株以10 μ g<2.0>和10 μ g <6.0>TCID<50>/鱼接种于比目鱼，分别产生60%和100%的死亡率。2000年和2001年，还从野生日本马鲛鱼（141条鱼）、暗带石斑鱼（101条鱼）和其他海洋鱼类（7种134条鱼）中进行了病毒分离试验，但仅从其中一些鱼类中分离到水病毒。

项目成果

Isshiki: "An outbreak of VHSV (viral hemorrhagic septicemia virus) infection in farmed Japanese flounder Paralichthys olivaceus in Japan"Diseases of Aquatic Organisms. 47. 87-99 (2001)

Isshiki：“日本养殖的牙鲽牙鲆爆发 VHSV（病毒性出血性败血症病毒）感染”水生生物疾病。

Takano: "Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild Japanese flounder, Paralichthys olivaceus"Bulletin of the Enropean Association of Fish Pathology. 20. 186-192 (2000)

Takano：“从野生日本牙鲆、Paralichthys olivaceus 中分离出病毒性出血性败血症病毒 (VHSV)”Enropean 鱼类病理学协会通报。

Takano R, Mori K, Nishizawa T, Arimoto M, and Muroga K: "Isolation of viruses from wild Japanese flounder"Fish Pathology. 36. 153-160 (2001)

Takano R、Mori K、Nishizawa T、Arimoto M 和 Muroga K：“从野生日本比目鱼中分离病毒”鱼类病理学。

Nishizawa: "Genetic relatedness among Japanese, American and European isolates of viral hemorrhagic septicemia virus(VHSV)"Diseases of Aquatic Organisms. 48(印刷中). (2002)

Nishizawa：“日本、美国和欧洲病毒性败血症病毒 (VHSV) 分离株的遗传相关性”水生生物疾病 48（出版中）。

Takano: "Isolation of viruses from wild Japanese flounder Paralichthys olivaceus"Fish Pathology. 36. 153-160 (2001)

Takano：“从野生日本牙鲽牙鲆中分离病毒”鱼类病理学。