ISOLATION OF ALGICIDAL SUBSTANCES IN JELLY-FISH AND THEIR UTILITY FOR REDUCTION OF RED-TIDES

水母中杀藻物质的分离及其减少赤潮的用途

• 批准号: 12660192
• 负责人: HIROMI Juro
• 金额: $ 2.24万
• 依托单位: NIHON UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660192/
• 关键词: JELLYFISH; AUTOLYSATE; ALGICIDES; POLYENOICFATTY ACIDS; Heterosigma akashiwo; Heterocapsa circularisquama; PEARL OYSTER; COLLAPSE OFRED-TIDES; 赤潮藻類; 高度不飽和脂肪酸

Algicidal substances to a harmful flagellate, Heterosigma akashiwo, in the autolysates of two species of jellyfish, Carybdea rastoni and Chrysaora melanester, were isolated by sequential procedures consisting of Bligh-Dyer's method, silica gel-TLC, and HPLC on a gel filtration column (Superdex peptide HR10/30) and on reverse phase column (L-column, ODS). Judging from the chromatographic behavior and LC-MS analysis of the isolated substances, it was suggested that five kinds of algicides from C. rastoni were Docosahexanoic acid (M : W=328, 22 : 6), Eicosapentaenoic acid (M.W=302, 20 : 5), Heptadecamonoenoic acid (M.W=268, 17 : 1), Triacylglycerols with M.W of 344 (C number of R1+R2+R3 was 13 and 7 of double bond) and 346 (C number of R1+R2+R3 was 13 and 6 of double bond), and that an algicide from C. melanaster was Tetradecsaheptaenoic acid (M.W=227, 15 : 7).A toxic flagellate Heterocapsa circularisquama is known to kill shellfish, such as oyster and a mother-of-pearl. The test was conducted to know whether the algicide (cis-5, 8, 11, 14, 17-icosapentanoic acid) of the jellyfish Aurelia aurita can kill toxic flagellate cells within oyster and a mother-of-pearl. Number of cells of H. circularisquama in the two shellfish species soaked in seawater added with autolysate of the jellyfish (final concentration ; 5 % v/v) decreased to 10-40 % of the initial number after 8 hours and to 5 % of the initial after 13 hours, without killing no shellfish during experiments. The present treatment is expected to utilize for reducing propagation of shellfishes infected with toxic flagellates.

本文采用Bligh-Dyer法、硅胶薄层层析和高效液相色谱法，在凝胶过滤柱（Superdex peptide HR 10/30）和反相柱（L-column，ODS）上分离了两种海蜇（Carybdea rastoni和Chrysaora melanester）自溶物中对有害鞭毛虫赤潮异弯藻（Heterosigma akashiwo）的杀藻活性物质。从色谱行为和分离物质的LC-MS分析结果来看，五种杀藻剂分别来自于C. rastoni是二十二碳六烯酸（M：W=328，22：6），二十碳五烯酸（M.W=302，20：5），十七碳一烯酸（男、女=268，17：1）、分子量为344的甘油三酯（R1+R2+R3的C数分别为13和7）和346（R1+R2+R3的C数分别为13和6）。已知有毒鞭毛虫Heterocapsa circulatelyquama杀死贝类，如牡蛎和珍珠母。为了解大耳海蜇（奥雷利亚aurita）杀藻剂（顺-5，8，11，14，17-二十碳五烯酸）对牡蛎和珍珠母体内有毒鞭毛虫细胞的杀灭效果，进行了实验研究。H.浸泡在添加有水母自溶物（最终浓度：5%v/v）的海水中的两种贝类中的环壳贝类在8小时后减少到初始数量的10- 40%，在13小时后减少到初始数量的5%，在实验期间没有杀死贝类。本发明的处理预期用于减少感染有毒鞭毛虫的贝类的繁殖。

项目成果

Uchida,N.,and Hiromi,J.: "Isolation and identification of algicides in a Jellyfish, Aurelia aurita"Fisheries Science.

Uchida, N. 和 Hiromi, J.：“Aurelia aurita 水母中除藻剂的分离和鉴定”渔业科学。

Hiromi,J.,and Uchida,N.: "Algicidal effect of IPA on a toxic phytoplankton, Alexandrium catenella"Fisheries Science.

Hiromi, J. 和 Uchida, N.：“IPA 对有毒浮游植物链状亚历山大藻的杀藻作用”渔业科学。