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Effect of different type of mitochondrial DNA on germline-differentiating potency of ES cell line in the mouse

不同类型线粒体DNA对小鼠ES细胞系种系分化能力的影响

基本信息

批准号：
12660254
负责人：
NAGAO Yasumitsu
金额：
$ 2.18万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

To examined the effect of mitochondrial DNA (mtDNA) on the germline-differentiating potency of ES cell line in the mouse, we investigated using the mice from standard and the mtDNA-congenic strain carrying different type of mtDNA under the same nuclear genetic background. We established the ES cell lines from standard strain C57BL/6J (B6) carring Mus musculus domesticus type mtDNA and from mtDNA-congenic strain C57BL/6Jmt^<spr> (B6 mt^<spr>) carring Mus spretus type mtDNA. When the ES cell lines derived from B6 and from B6 mt^<spr> were used to compare the germline-differentiating potency in the chimeric mice with ICR strain embryos, the germline-chimera mouse was obtained from only the B6 mt^<spr> ES cell line. We examined totipotency of the ES lines by tetraploid complemention. The tetraprloid-chimera mice were obtained from B6 ES cell line and from B6 mt^<spr> ES cell lines. From these results, we thought that the sperm derived from B6 mt^<spr> ES cell had high capacity of fertilization. When we compared B6 sperm with B6 mt^<spr> sperm by capacity of fertilization in vitro fertilization, B6 mt^<spr> sperm was higher than B6 sperm. The capacity of sperm from another mtDNA-congenic strain BALB/c mt^<mus> carring Mus musculus musculus type mtDNA was lower than the capacity of B6 sperm. The germ-line chimera mice were produced when B6 ES cells were injected into the blastosysts obtained from BALB/c mt^<mus>. It was suggested that mtDNA influenced the capacity of sperm from ES cell.

为了研究线粒体DNA （mtDNA）对小鼠胚胎干细胞分化能力的影响，我们在相同的核遗传背景下，用携带不同类型mtDNA的标准小鼠和携带不同mtDNA基因的小鼠进行了研究。以携带家鼠型mtDNA的标准菌株C57BL/6J （B6）和携带家鼠型mtDNA的mtDNA基因菌株C57BL/6Jmt^<spr> (B6 mt^<spr>)分别建立ES细胞系。用B6和B6 mt^<spr>来源的ES细胞系比较ICR株胚胎嵌合小鼠的种系分化能力时，只从B6 mt^<spr>来源的ES细胞系获得了种系嵌合小鼠。我们通过四倍体互补检测ES系的全能性。四倍体嵌合体小鼠分别来自B6 ES细胞系和B6 mt^<spr> ES细胞系。从这些结果来看，我们认为来自B6 mt^<spr> ES细胞的精子具有较高的受精能力。体外受精比较B6精子与B6 mt^<spr>精子的受精率，B6 mt^<spr>精子的受精率高于B6精子。携带小家鼠型mtDNA的另一mtDNA同源菌株BALB/c mt^<mus>的精子容量低于B6精子容量。将B6 ES细胞注射到BALB/c mt^<mus>获得的胚系中，产生了种系嵌合体小鼠。提示mtDNA影响胚胎干细胞精子的功能。