Effect of different type of mitochondrial DNA on germline-differentiating potency of ES cell line in the mouse

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To examined the effect of mitochondrial DNA (mtDNA) on the germline-differentiating potency of ES cell line in the mouse, we investigated using the mice from standard and the mtDNA-congenic strain carrying different type of mtDNA under the same nuclear genetic background. We established the ES cell lines from standard strain C57BL/6J (B6) carring Mus musculus domesticus type mtDNA and from mtDNA-congenic strain C57BL/6Jmt^<spr> (B6 mt^<spr>) carring Mus spretus type mtDNA. When the ES cell lines derived from B6 and from B6 mt^<spr> were used to compare the germline-differentiating potency in the chimeric mice with ICR strain embryos, the germline-chimera mouse was obtained from only the B6 mt^<spr> ES cell line. We examined totipotency of the ES lines by tetraploid complemention. The tetraprloid-chimera mice were obtained from B6 ES cell line and from B6 mt^<spr> ES cell lines. From these results, we thought that the sperm derived from B6 mt^<spr> ES cell had high capacity of fertilization. When we compared B6 sperm with B6 mt^<spr> sperm by capacity of fertilization in vitro fertilization, B6 mt^<spr> sperm was higher than B6 sperm. The capacity of sperm from another mtDNA-congenic strain BALB/c mt^<mus> carring Mus musculus musculus type mtDNA was lower than the capacity of B6 sperm. The germ-line chimera mice were produced when B6 ES cells were injected into the blastosysts obtained from BALB/c mt^<mus>. It was suggested that mtDNA influenced the capacity of sperm from ES cell.