Reduction of total manure excretion from livestock using by fiber degradation enzymes

以纤维素酶和其他纤维降解酶为底物，对纯纤维素、纯净饲料和商品饲料的纤维素消化率进行了体外研究。另外，还进行了植酸酶对纤维素和磷利用的体外研究。通过实验的方式，研究了几种酶对大鼠消化液中纤维素的降解率和纤维素的降解率。通过测定干物质和营养物质的消化率，同时研究了能量利用效率和通过增加纤维素消化减少粪便排泄量。研究了日粮中添加纤维素对雏鸡、成年小型猪和生长猪粪便、干物质消化率和纤维素消化率的影响。此外，还研究了植酸酶和纤维素酶在家禽和小型猪中的联合作用。体外研究表明，在盐酸-胃蛋白酶缓冲溶液中，一些复合酶降低了纤维素的降解，植酸酶的联合利用降低了纤维素的降解率。有些酶能减少粪便排泄量，提高干物质消化率，但对粗纤维(木质素、纤维素)的消化率影响不大。这一结果导致了一种猜测，即这些酶提高了可溶性氮TEE提取物的消化率。部分纤维素添加剂降低了植酸的降解，降低了植酸酶的活性，同时添加了植酸酶。人们认为，联合补充纤维素和植酸酶有时并不有效。

In vitro studies were done to evaluate a cellulose digestibility of pure cellulose, purified diet and commercial diet as substrate by using cellulase including other fiber degradation enzymes. Another in vitro studies were done to examine the effect of phytase on the utilization of cellulose and phosphorus.In a way of experiments, cellulose degradability in the digestive tract and an efficiency of cellulose degradation were studied after the collection of digesta from rats given some kinds of enzyme. An efficiency of energy utilization and a reduction of fecal excretion by increasing cellulose digestion were simultaneously studied by measuring dry matter and nutrients digestibilities. The effect of cellulose supplementation to diets on fecal amount, dry matter digestibility and cellulose digestibility was studied in poultries, adult mini-pigs and growing pigs. A combined effect of phytase and cellulose was also studied in poultries and mini-pigs.The in vitro study under HCl-pepsin buffer solution showed that some complex enzymes reduced cellulose degradation and the combined utilization of phytase reduced cellulose degradability efficiency. Some enzymes reduced the amount of excreted feces and improved dry matter digestibihty, however the enzymes seemed to have little effect on the digestibility of crude fiber (lignin, cellulose). The result leads to a speculation that the enzymes improve the digestibility of soluble nitrogen tee extract. Some cellulose supplements reduced phytic acid degradation and made an action to decrease phytase activity which was supplemented simultaneously. It is considered that a combined supplementation of cellulose and phytase is sometimes not effective.