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CHANGES OF PARATHYROIDS AND BONE TISSUES IN AGE-RELATED OSTEOPOROSIS: WITH SPECIAL REGERENCES TO PTHrP and AGEs.

年龄相关性骨质疏松症中甲状旁腺和骨组织的变化：特别关注 PTHrP 和 AGE。

基本信息

批准号：
12670187
负责人：
MATSUSHITA Hitoshi
金额：
$ 2.05万
依托单位：
Okinaka Memorial Institute for Medical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670187/
关键词：
AGE RAGE Parathyroid Lung Neoplasm Hyperplasia Hmodialysis cyclin D parathyroid hyperplasia adenoma

项目摘要

Advanced Glycation End Products (AGEs) are produced by the non-enzymatic reduction of amino-acids (Mallard reaction, late stage).lt is well known that the production of AGEs are remarkably increased in the patients with long-standing renal failure under the control of hemodialysis. We suspect the renal osteodystrophy in long-term hemodialysis cases may have some relationship with increased production of AGEs. Therefore, we made the hypothesis that the over-expression of the receptor for AGEs (RAGE) may occur in both parathyroid glands and bone tissue. This research project was performed as follows with two steps researches.First, we examined the expression of AGEs in parathyroid glands and bone tissues. AGEs expression was immunohistochemically examined in normal parathyroids (nPTG), secondary parathyroid hyperplasia (HPT), parathyroid adenoma (PTA). As a result, AGEs were expressed all examined parathyroid tissues in both normal and pathologic cases. There was a strong AGEs expression in the area of oxyphil cells. Because oxyphil cells in parathyroids are shown tobe less active cells both in hormone production and cell proliferation, AGEs expression of parathyroid glands also related to the cell aging. However, there was no RAGE expression in parathyroid glands.Second, Rage expression was examined in Ilug tissues as control study. Rage was diffusely expressed in type I pneumoctes. It is of great interest that the tumor cell in adenomatous lesion was positive contrasted to the nagative reaction of tumor cells in the very well differentiated adenocarcinoma in the lung neoplasms.

晚期糖基化终产物（AGEs）是由氨基酸的非酶还原产生的（绿头鸭反应，后期）。众所周知，长期肾功能衰竭患者在血液透析控制下，age的产生显著增加。我们怀疑长期血液透析患者的肾性骨营养不良可能与AGEs的增加有关。因此，我们假设AGEs受体（RAGE）的过表达可能发生在甲状旁腺和骨组织中。本研究项目分为两步研究。首先，我们检测了AGEs在甲状旁腺和骨组织中的表达。免疫组化法检测AGEs在正常甲状旁腺（nPTG）、继发性甲状旁腺增生（HPT）、甲状旁腺瘤（PTA）组织中的表达。结果，在正常和病理病例的甲状旁腺组织中，AGEs均有表达。在嗜氧细胞区有较强的AGEs表达。由于甲状旁腺的氧细胞在激素产生和细胞增殖方面都是较不活跃的细胞，甲状旁腺的AGEs表达也与细胞衰老有关。而RAGE在甲状旁腺中未见表达。其次，在Ilug组织中检测Rage表达作为对照研究。Rage在I型肺炎中弥漫性表达。腺瘤病变中肿瘤细胞呈阳性反应，而肺肿瘤中分化非常好的腺癌中肿瘤细胞呈阴性反应，这一点引起了人们的极大兴趣。