Studies on mast cell hyperplasia induced by nematode infection and E-cadherin and Integrin αE

肥大细胞通过与组织成分的粘附相互作用定位于各种组织中。细胞粘附分子被认为在包括肠粘膜在内的组织内的细胞迁移和定位中起关键作用。结果发现，在IL-3和IL-4培养基中培养的骨髓源性肥大细胞（BMMC）表达E-cadherin和钙粘附素相关的胞浆蛋白α-、β-、p120-catenin。集落实验和细胞粘附实验证实，E-钙粘蛋白与肥大细胞分化和细胞间相互作用的某些阶段的调节有关。接下来，为了阐明肠上皮内相关细胞粘附分子如E-cadherin和整合素αE（CD 103）β7的表达，使用在含有IL-3、IL-9、干细胞因子（SCF）和转化生长因子-β1（TGF-β1）的生长培养基中培养的BMMC作为肠粘膜肥大细胞（IMMC）的体外类似物。小鼠肥大细胞蛋白酶-1（mMCP-1）的表达，只有IMMC诱导BMMC补充这些生长因子。粘膜型肥大细胞中整合素αE、β7亚基表达明显。这些结果提示，肥大细胞上表达的E-cadherin及其异源配体整合素αEβ7可能通过与E-cadherin的异型或同型相互作用，在肥大细胞-上皮细胞粘附和/或锚定在肠上皮中发挥作用。

Mast cells localize in various tissues through adhesive interactions with tissue components. Cell adhesion molecules are thought to play a critical role in cell migration and localization within tissues including gut mucosa. We found the expression of E-cadherin and cadherin-associated cytoplasmic proteins such as α-, β-, p120-catenin in bone marrow-derived mast cells (BMMC) cultured in interleukin-3 (IL-3) and IL-4 rich medium. E-cadherin was associated with the regulation of certain stages of mast cells differentiation and cell-cell interaction confirmed by colony assay and cell adhesion assay. Next, to clarify the expression of gut intraepithelium-related cell adhesion molecules such as E-cadherin and integrin αE (CD103)β7, BMMC cultured in the growth medium containing with IL-3, IL-9, stem cell factor (SCF), and transforming growth factor-β1 (TGF-β1 were used an in vitro analog of intestinal mucosal mast cells (IMMC). Mouse mast cell protease-1 (mMCP-1) expressed on only IMMC was induced in BMMC supplemented with these growth factors. Significant expression of integrin αE, and β7 subunit were observed in cultured mucosal type mast cells. E-cadherin was also expressed on these mast cells.These results suggest that E-cadherin and its heteroligand integrin αEβ7 expressed on mast cells might play a role in mast cell-epithelial cell adhesion and/or remaining anchored at the intestinal epithelia by heterotypic or homotypic interacting with E-cadherin.