Studies on mast cell hyperplasia induced by nematode infection and E-cadherin and Integrin αE

线虫感染引起的肥大细胞增生与E-cadherin和Integrin αE的研究

• 批准号: 12670237
• 负责人: TEGOSHI Tatsuya
• 金额: $ 1.86万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670237/
• 关键词: mast sell; Cyto adherence molecule; Nematode infection; E-cadherin; Integrin αE; 培養; カドヘリン; CD103

Mast cells localize in various tissues through adhesive interactions with tissue components. Cell adhesion molecules are thought to play a critical role in cell migration and localization within tissues including gut mucosa. We found the expression of E-cadherin and cadherin-associated cytoplasmic proteins such as α-, β-, p120-catenin in bone marrow-derived mast cells (BMMC) cultured in interleukin-3 (IL-3) and IL-4 rich medium. E-cadherin was associated with the regulation of certain stages of mast cells differentiation and cell-cell interaction confirmed by colony assay and cell adhesion assay. Next, to clarify the expression of gut intraepithelium-related cell adhesion molecules such as E-cadherin and integrin αE (CD103)β7, BMMC cultured in the growth medium containing with IL-3, IL-9, stem cell factor (SCF), and transforming growth factor-β1 (TGF-β1 were used an in vitro analog of intestinal mucosal mast cells (IMMC). Mouse mast cell protease-1 (mMCP-1) expressed on only IMMC was induced in BMMC supplemented with these growth factors. Significant expression of integrin αE, and β7 subunit were observed in cultured mucosal type mast cells. E-cadherin was also expressed on these mast cells.These results suggest that E-cadherin and its heteroligand integrin αEβ7 expressed on mast cells might play a role in mast cell-epithelial cell adhesion and/or remaining anchored at the intestinal epithelia by heterotypic or homotypic interacting with E-cadherin.

肥大细胞通过与组织成分的粘附相互作用定位于各种组织中。细胞粘附分子被认为在包括肠粘膜在内的组织内的细胞迁移和定位中发挥着关键作用。我们发现在富含白介素 3 (IL-3) 和 IL-4 的培养基中培养的骨髓源性肥大细胞 (BMMC) 中存在 E-钙粘蛋白和钙粘蛋白相关细胞质蛋白（例如 α-、β-、p120-连环蛋白）的表达。集落测定和细胞粘附测定证实，E-钙粘蛋白与肥大细胞分化的某些阶段和细胞间相互作用的调节有关。接下来，为了阐明肠上皮内相关细胞粘附分子如E-钙粘蛋白和整合素αE(CD103)β7的表达，在含有IL-3、IL-9、干细胞因子(SCF)和转化生长因子-β1(TGF-β1)的生长培养基中培养的BMMC使用肠粘膜肥大细胞(IMMC)的体外类似物。 仅在 IMMC 上表达的肥大细胞蛋白酶-1 (mMCP-1) 在补充了这些生长因子的 BMMC 中被诱导。在培养的粘膜型肥大细胞中观察到整合素αE和β7亚基的显着表达。 E-cadherin也在这些肥大细胞上表达。这些结果表明，肥大细胞上表达的E-cadherin及其异配体整合素αEβ7可能在肥大细胞中发挥作用。 细胞-上皮细胞粘附和/或通过与E-钙粘蛋白的异型或同型相互作用保持锚定在肠上皮上。

项目成果

Tegoshi T.: "E-cadherin and cadherin-associated cytoplasmic protein are expressed in murine mast cells"Laboratory Investigation. 80. 1571-1581 (2000)

Tegoshi T.：“E-钙粘蛋白和钙粘蛋白相关细胞质蛋白在小鼠肥大细胞中表达”实验室研究。

Tegoshi T et al.: "E-cadherin and cadherin-associated cytoplasmic protein are expressed in marine mast cells"raboratory Investigation. 80. 1571-1581 (2000)

Tegoshi T 等人：“E-钙粘蛋白和钙粘蛋白相关细胞质蛋白在海洋肥大细胞中表达”实验室研究。

Tegoshi T, Nisda M et al.: "E-cadherin and cudherin-associated cytoplasmic protein are expressed in murine mast cells"Laboratory Investigation. 80・10. 1571-1581 (2000)

Tegoshi T、Nisda M 等人：“E-钙粘蛋白和 Cudherin 相关细胞质蛋白在小鼠肥大细胞中表达”实验室研究 80・10（2000）。