Studies on mast cell hyperplasia induced by nematode infection and E-cadherin and Integrin αE

肥大细胞通过与组织成分的黏附作用定位于各种组织中。细胞黏附分子被认为在细胞在包括肠粘膜在内的组织内的迁移和定位中起关键作用。我们发现在白介素3(IL-3)和IL-4富含的培养液中培养的骨髓肥大细胞表达E-钙粘素和钙粘素相关胞浆蛋白α-，β-、p120-连环蛋白。集落形成实验和细胞黏附实验证实E-钙粘素参与了肥大细胞分化的某些阶段的调控和细胞与细胞的相互作用。为了明确肠上皮内相关细胞黏附分子E-钙粘素和整合素αE(CD103)β7的表达，采用体外模拟肠粘膜肥大细胞的方法，在含有IL-3、IL-9、干细胞因子和转化生长因子-β-1(转化生长因子-β-1)的培养液中进行培养。仅在IMMC上表达的小鼠肥大细胞蛋白-1(mMCP-1)在添加这些生长因子的BMMC中被诱导。在培养的粘膜型肥大细胞中可观察到整合素αE和β7亚单位的显著表达。这些结果提示，肥大细胞上表达的E-钙粘蛋白及其异型整合素αEβ7可能通过与E-钙粘附素的异型或同型相互作用，在肥大细胞与肠上皮细胞的黏附和/或锚定中发挥作用。

Mast cells localize in various tissues through adhesive interactions with tissue components. Cell adhesion molecules are thought to play a critical role in cell migration and localization within tissues including gut mucosa. We found the expression of E-cadherin and cadherin-associated cytoplasmic proteins such as α-, β-, p120-catenin in bone marrow-derived mast cells (BMMC) cultured in interleukin-3 (IL-3) and IL-4 rich medium. E-cadherin was associated with the regulation of certain stages of mast cells differentiation and cell-cell interaction confirmed by colony assay and cell adhesion assay. Next, to clarify the expression of gut intraepithelium-related cell adhesion molecules such as E-cadherin and integrin αE (CD103)β7, BMMC cultured in the growth medium containing with IL-3, IL-9, stem cell factor (SCF), and transforming growth factor-β1 (TGF-β1 were used an in vitro analog of intestinal mucosal mast cells (IMMC). Mouse mast cell protease-1 (mMCP-1) expressed on only IMMC was induced in BMMC supplemented with these growth factors. Significant expression of integrin αE, and β7 subunit were observed in cultured mucosal type mast cells. E-cadherin was also expressed on these mast cells.These results suggest that E-cadherin and its heteroligand integrin αEβ7 expressed on mast cells might play a role in mast cell-epithelial cell adhesion and/or remaining anchored at the intestinal epithelia by heterotypic or homotypic interacting with E-cadherin.