Colonization factor and protective antigen of Vibrio cholerae O1

霍乱弧菌O1定植因子和保护性抗原

• 批准号: 12670260
• 负责人: IWANAGA Masaaki
• 金额: $ 1.98万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670260/
• 关键词: Vibrio cholerae; type IV pili; structure gene; adhesin; Bacteria in vivo; protective antigen; Vibrio cholerae; 定着因子

To identify adhesive factor of Vibrio cholerae to the intestinal epithelium, the relation between V14 pili (adhesive) and cholera vibrios were investigated. Although V14 pilus was not expressed on the surface of cholera vibrios, its antigen was detected in the cytoplasm. The gene analysis of major subunit pilin (vcfA, or mshA in cholera vibrio) started from the N-terminal amino acid sequence of the protein. N-terminal sequence of vcfA was highly homologous to mshA, but the C-terminal sequence was independent. Since Δ vcfA strain was equally adhesive to Hep2 cells, whole structural operon of V14 pilus was investigated. After all, it was clarified that the gene clusters of MSHA pili and V14 pili of a V. cholerae O34 strain had 7 ORFs, and that the ORFs were almost identical between the two, except C-terminal sequence of mshA and N-terminal of mshQ, and that the pili consisted of 3 subunit proteins (a major pilin and 2 minor pilins), and that mshQ encodes outer membrane protein which is responsible for the biogenesis of pili. From these results, V14 pilus was regarded as a variant of MSHA pilus of V. cholerae O1, and is not involved in adhesion.

为确定霍乱弧菌对肠上皮细胞的粘附因子，研究了霍乱弧菌V14皮利（粘附因子）与霍乱弧菌的关系。虽然霍乱弧菌表面不表达V14菌毛，但在胞浆中检测到其抗原。菌毛蛋白主要亚基（vcfA，或霍乱弧菌中的mshA）的基因分析从蛋白质的N-末端氨基酸序列开始。vcfA的N端序列与mshA高度同源，而C端序列是独立的。由于Δ vcfA菌株对Hep 2细胞具有相同的粘附性，因此研究了V14菌毛的整个结构操纵子。毕竟，澄清了霍乱弧菌O 34株MSHA皮利和V14皮利的基因簇有7个ORF，并且除了mshA的C末端序列和mshQ的N末端序列之外，两者之间的ORF几乎相同，并且皮利由3个亚基蛋白组成（一种主要菌毛蛋白和2种次要菌毛蛋白），并且mshQ编码负责皮利生物发生的外膜蛋白。根据这些结果，V14菌毛被认为是霍乱弧菌O 1的MSHA菌毛的变体，并且不参与粘附。

项目成果

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Toma C.：“与霍乱弧菌血凝素/蛋白酶相关的豚鼠气单胞菌金属蛋白酶的纯化和表征。”FEMS Microbiol.Lett.. 170. 237-242 (1999)

Nakasone N.: "Filamentous phage fs1 of Vibrio cholerae O139."Microbiol.Immunol.. 42(3). 237-239 (1998)

Nakasone N.：“霍乱弧菌 O139 的丝状噬菌体 fs1。”Microbiol.Immunol.. 42(3)。

Tomoko Miyazato: "Molecular analysis of VcfQ protein involved in Vibrio cholerae type IV pilus biogenesis"J.Med.Microbiol.. 52(4). 283-288 (2003)

Tomoko Miyazato：“参与霍乱弧菌 IV 型菌毛生物发生的 VcfQ 蛋白的分子分析”J.Med.Microbiol.. 52(4)。

Nakasone N.: "A filamentous phage of Vibrio parahaemolyticus O3 : K6 isolated in Laos"Microbiol.Immunol.. 43(4). 385-388 (1999)

Nakasone N.：“副溶血弧菌 O3 的丝状噬菌体：在老挝分离的 K6”Microbiol.Immunol.. 43(4)。

Hiromi Kuroki: "Gene analysis of Vibrio cholerae NAGV14 pilus and its distribution"Microbiol.Immunol.. 45(6). 417-424 (2001)

Hiromi Kuroki：“霍乱弧菌 NAGV14 菌毛的基因分析及其分布”Microbiol.Immunol.. 45(6)。