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Distribution of Exposed Organic Solvent in the Brain and its Effect on GABA_A Receptor

Distribution of Exposed Organic Solvent in the Brain and its Effect on GABA_A Receptor
暴露有机溶剂在脑内的分布及其对GABA_A受体的影响
批准号:
12670356
负责人:
ITO Takehiko
金额:
$1.66万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2000
资助国家:
日本
项目状态:
已结题
起止时间:
2000 至 2001

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项目成果

ITO Takehiko的其他基金

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中文摘要
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英文摘要
1. Establishment of a new system for exposing organic solvents to small animalsA system suitable for exposing m-xylene to rats was made, and a series of experiments was conducted using this system. The exposure system was easy to handle, and was useful.2. Effects of m-xylene exposure on GABA_A receptor in the brainAutoradiographic study was done using three ligands specific to GABA_A receptors. Autoradiography using [^<35>S]t-butylbicyclophosphorothionate revealed a significant increase in the binding in the cerebellum, whereas that using [^3H]flunitrazepam showed no significant changes. To further investigate the possibility of a receptor subunit-specific effect of m-xylene, autoradiogram using [^3H]Ro15-4513 was obtained. Significant changes in binding were found in the II-IIIth layer of the cerebral cortex, CA1, Inferior colliculus and in the granule cells of the cerebellum. The increase in the binding in the granule cells seemed to be a subunit-specific change, possibly due to alteration in alpha6 subunit-containing receptors.3. Measurement of m-xylene concentration in several areas of the brainThe levels of m-xylene on and after the exposure were measured, and referenced with its metabolite concentration in urine.4. Measurement of m-methylhippuric acid in urine on and after the exposureThe levels of m-methylhippuric acid were measured on and after the exposure. While m-xylene level and m-methylhippuric acid level paralleled during the exposure, the two levels decreased in a different manner after the exposure.These findings taken together, the basic data to develop a new biological effect monitoring of m-xylene were obtained. The new biological monitoring method will use changes in GABA_A receptor as indices of biological effect, and metabolite of m-xylene in urine (m-methylhippuric acid and/or its precursor m-tolualdehyde) as indices of the exposure, respectively.
期刊论文(4)
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会议论文
Ito, T., Yoshitome, K., Horike, T. and Kira S.: "Distribution of inhaled m-xylene in rat brain and its effect on GABA_A receptor binding"Journal of Occupational Health. 44. 69-75 (2002)
Ito, T.、Yoshitome, K.、Horike, T. 和 Kira S.:“吸入间二甲苯在大鼠脑中的分布及其对 GABA_A 受体结合的影响”职业健康杂志。
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作者: []
通讯作者:
Ito T.,Yoshitome K., Nakayama S.,Kira S. and Horike T.: "Effect of m-xylene on [35S]t-butylbicyclophosphorothionate binding in the rat brain"Soc.Neurosci.Abstr. 26(2). 2080 (2000)
Ito T.、Yoshitome K.、Nakayama S.、Kira S. 和 Horike T.:“间二甲苯对大鼠脑中[35S]叔丁基二环硫代磷酸酯结合的影响”Soc.Neurosci.Abstr。
DOI: --
发表时间:
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作者: []
通讯作者:
Ito T., Yoshitome K., Horike T., Kira S.: "Distribution of inhaled m-xylene in rat brain and its effect on GABAA receptor binding"Journal of Occupational Health. 44. 69-75 (2002)
Ito T.、Yoshitome K.、Horike T.、Kira S.:“吸入间二甲苯在大鼠脑中的分布及其对 GABAA 受体结合的影响”职业健康杂志。
DOI: --
发表时间:
期刊:
影响因子: --
作者: []
通讯作者:
Ito T., Yoshitome K., Horike T., Kira S.: "Distribution of inhaled m-xylene in rat brain and its effect on GABAA receptor binding"Journal of Occupational Health. 44(印刷中). (2002)
Ito T.、Yoshitome K.、Horike T.、Kira S.:“吸入间二甲苯在大鼠脑中的分布及其对 GABAA 受体结合的影响”《职业健康杂志》44(出版中)。
DOI: --
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作者: []
通讯作者:
Coexistence mechanism analysis of different migration strategies of grassland mammals and detection of hormones inducing migration
Detection of important areas for wildlife conservation in Mongolia incorporating environmental predictability and heterogeneity
  • 批准号:
    15K06931
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $3.24万
  • 财政年份:
    2015
  • 负责人:
    ITO Takehiko
  • 依托单位:
Conservation of great migration of wild mammals in Mongolia: monitoring of current conditions and assessment of new railroad construction
  • 批准号:
    24510326
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $3.49万
  • 财政年份:
    2012
  • 负责人:
    ITO Takehiko
  • 依托单位:
Basic study on risk analysis within a school district using small area analysis
  • 批准号:
    19500578
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 资助金额:
    $1.5万
  • 财政年份:
    2007
  • 负责人:
    ITO Takehiko
  • 依托单位: