Localigation oi P.acnes in sarcoid granumoma and identification of a liability antigen in P.acnes cell-wall component

肉芽肿中痤疮丙酸杆菌的定位和痤疮丙酸杆菌细胞壁成分中的责任抗原的鉴定

• 批准号: 12670425
• 负责人: NAKATA Yasunari
• 金额: $ 1.86万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670425/
• 关键词: sarcoidosis; Propionibacterium acnes; lymphocyte; サイコイドーシス; プロピオニバクテリウムーアクネス

Despite the extensive investigations into the pathogenesis of sarcoidosis, the etiology of sarcoidosis is still unknown. In previous studies, Honma H. and his colleagues found Propionibacterium acnes (P. acnes) in the affected lymph nodes of sarcoidosis patients. In this study, we investigated to detect DNA of P. acnes in cells recovered from bronchoalveolar lavage (BAL) in patients with sarcoidosis. We applied a nested polymerase chain reaction (PCR) assay and in situ PCR hybridization as a tool to detect DNA of P. acnes. The PCR-technique is based on the amplification of P. acnes small subunit ribosomal RNA. BAL cells from thirty patients with histologically proven sarcoidosis and 30 controls with other pulmonary diseases were underwent assay by specifically detected DNA from P. acnes. DNA of P. acnes was detected in 70% in the BAL cells with sarcoidosis patients and 23.3% in the controls, the proportion of sarcoidosis is significantly greater than that of controls (p<0.001). By in situ PCR hybridization, signal of P. acnes DNA was detected only in cytoplasm of alveolar macrophages from sarcoidosis, but not from non-sarcoidosis patients.

尽管对结节病的发病机制进行了广泛的研究，但结节病的病因仍不清楚。在以往的研究中，Honma H.和他的同事在结节病患者的受累淋巴结中发现了痤疮丙酸杆菌。在这项研究中，我们研究了检测痤疮丙酸杆菌的DNA从支气管肺泡灌洗（BAL）的结节病患者的细胞。我们应用巢式聚合酶链反应（PCR）和原位PCR杂交作为检测痤疮丙酸杆菌DNA的工具。PCR技术基于痤疮丙酸杆菌小亚基核糖体RNA的扩增。用痤疮丙酸杆菌特异性DNA对30例经病理证实的结节病患者和30例其他肺部疾病对照者的BAL细胞进行检测。在结节病患者的BAL细胞中，痤疮丙酸杆菌DNA的阳性率为70%，对照组为23.3%，结节病患者的阳性率明显高于对照组（P<0.001）。通过原位PCR杂交，仅在结节病患者肺泡巨噬细胞的细胞质中检测到痤疮丙酸杆菌DNA信号，而在非结节病患者的细胞质中未检测到痤疮丙酸杆菌DNA信号。

项目成果

Mikio KATAOKA et al.: "Response of BAL cells to Pripionibacterium acnes in sarcoidosis patients"The 7^<th> Wand Association of Sarcoidosis and Other Granulomatous Disorders. 36 (2002)

Mikio KATAOKA 等人：“结节病患者中 BAL 细胞对痤疮丙酸杆菌的反应”结节病和其他肉芽肿性疾病第 7 届 Wand Association。

中田 安成(分担執筆): "サルコイドーシス;サルコイドーシスの病理・病態生理"最新医学社. 9 (2002)

Yasunari Nakata（撰稿人）：“结节病；结节病的病理学和病理生理学”Shinsei Igakusha 9 (2002)。

Mikio KATAOKA et al.: "Response of BAL cells to Pripionibacterium acnes in sarcoidosis patients"The 7^<th> World Association of Sarcoidosis and Other Granulomatous Disorders. 36 (2002)

Mikio KATAOKA 等人：“结节病患者中 BAL 细胞对痤疮丙酸杆菌的反应”第七届世界结节病和其他肉芽肿性疾病协会。

Mikio KATAOKA et al: "Response of BAL cells to Pripionibacterium acnes in sarcoidosis patients"The 7^<th> World Association of Sarcoidosis and Other Granulomatous Disorders. 36. (2002)

Mikio KATAOKA 等人：“结节病患者中 BAL 细胞对痤疮丙酸杆菌的反应”第七届世界结节病和其他肉芽肿性疾病协会。