Localigation oi P.acnes in sarcoid granumoma and identification of a liability antigen in P.acnes cell-wall component

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Despite the extensive investigations into the pathogenesis of sarcoidosis, the etiology of sarcoidosis is still unknown. In previous studies, Honma H. and his colleagues found Propionibacterium acnes (P. acnes) in the affected lymph nodes of sarcoidosis patients. In this study, we investigated to detect DNA of P. acnes in cells recovered from bronchoalveolar lavage (BAL) in patients with sarcoidosis. We applied a nested polymerase chain reaction (PCR) assay and in situ PCR hybridization as a tool to detect DNA of P. acnes. The PCR-technique is based on the amplification of P. acnes small subunit ribosomal RNA. BAL cells from thirty patients with histologically proven sarcoidosis and 30 controls with other pulmonary diseases were underwent assay by specifically detected DNA from P. acnes. DNA of P. acnes was detected in 70% in the BAL cells with sarcoidosis patients and 23.3% in the controls, the proportion of sarcoidosis is significantly greater than that of controls (p<0.001). By in situ PCR hybridization, signal of P. acnes DNA was detected only in cytoplasm of alveolar macrophages from sarcoidosis, but not from non-sarcoidosis patients.