Enhancement of anti-allergic effect of DNA vaccine by macrolide antibiotic

大环内酯类抗生素增强DNA疫苗的抗过敏作用

• 批准号: 12670432
• 负责人: SATO Yukio
• 金额: $ 2.18万
• 依托单位: Fukushima Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670432/
• 关键词: DNA VACCINE; MCROLIDE ANTIBIOTIC; ALLERGY; EOSINOPHILIA

We examined the effect of erythromycin (EM), a 14-membered macrolide, on the immune response to a DNA vaccine that induces a T-helper-1 (Thl)-biased immune response through a Thl-promoting adjuvant effect of unmethylated CpG motifs within plasmid DNA. EM enhanced Thl responses in plasmid DNA-immunized mice as measured by antigen-specific IgG2a antibody production, interferon- γ production by antigen-specific CD4+ T cells, and cytotoxic T lymphocyte responses. Since immunization with plasmid DNA encoding a specific allergen via the systemic routes has been shown to both prevent activation of allergen-specific Th2 cells following injection of allergen protein in aluminum hydroxide, we attempted to determine whether EM enhances the inhibition of pulmonary eosinophilia in ovalbumin (OVA)-sensitized mice after inhalation OVA challenge. The results showed that EM enhances the inhibitory effect of DNA vaccine on pulmonary eosinophilia.

我们检查了红霉素 (EM)（一种 14 元大环内酯）对 DNA 疫苗免疫反应的影响，该疫苗通过质粒 DNA 内未甲基化 CpG 基序的促进 T-helper-1 (Thl) 的佐剂作用诱导 T-helper-1 (Thl) 偏向免疫反应。通过抗原特异性IgG2a抗体产生、抗原特异性CD4+T细胞产生干扰素-γ以及细胞毒性T淋巴细胞反应来测量，EM增强了质粒DNA免疫小鼠中的Th1反应。由于通过全身途径用编码特定过敏原的质粒 DNA 进行免疫已被证明可以防止注射氢氧化铝中的过敏原蛋白后过敏原特异性 Th2 细胞的激活，因此我们试图确定 EM 是否增强了卵清蛋白 (OVA) 致敏小鼠在吸入 OVA 攻击后对肺嗜酸粒细胞增多的抑制作用。结果表明，EM增强了DNA疫苗对肺嗜酸性粒细胞增多的抑制作用。

项目成果

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