THE STUDY OF THE EXPRESSIONS AND FUNCTIONS OF NUCLEAR HORMONE RECEPTORS IN HUMAN ALVEOLAR MACROPHAGES

人肺泡巨噬细胞核激素受体表达和功能的研究

基本信息

  • 批准号:
    12670555
  • 负责人:
  • 金额:
    $ 2.11万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2000
  • 资助国家:
    日本
  • 起止时间:
    2000 至 2001
  • 项目状态:
    已结题

项目摘要

(1) Expression of the 25-hydroxyvitamin D3 lα-hydroxylase gene in human alveolar macrophagesWe demonstrated expression of the 25-hydroxyvitamin D3 lα-hydroxylase (lαOHse) gene in human alveolar macrophages (AM) prepared from bronchoalveolar lavage cells by reverse transcription polymerase chain reaction (RT-PCR). The lα Hse-mRNA level measured by semiquantitative RT-PCR was five-fold higher in patients with sarcoidosis than control patients with other pulmonary disorders. There was a good correlation between the lα Ohse-mRNA level in bronchoalveolar lavage samples and the relative population of alveolar lymphocytes, the CD4/CD8 ratio, serum ACE, serum ionized calcium and the lα, 25-dihydroxylase D3/25-hydroxyvitamin D3 (25D3/1,25D3) ratio, suggesting that lα Ohse in AM was functional and significantly correlated with the activity of sarcoidosis and calcium metabolism.Among patients with various pulmonary diseases, we found that the lα Ohse-mRNA in patients with lung carcinomas was sign … More ificantly enhanced, about twice the control. Correlation was observed between lα Ohse-mRNA level and the 25D3/I,25D3 ratio. Though not statistically significant, the lα Ohse-mRNA level in AM tended to increase in the patients with advanced stage.(2) Expression and Function of Peroxisome Proliferator-Activated Receptor (PPAR) T in Human Alveolar MacrophagesUsing RT-PCR, we demonstrated the expression of PPAR mRNAs in freshly isolated human AM. PPARα, β and γ were all expressed, but PPARγ was dominant. A specific ligand of PPARγ, 15-deoxy-D12,14prostaglandin J2 (15d-PGJ2), significantly decreased LPS-induced TNF-α production by Ams and markedly increased the expression of CD36 that mediates the phagocytosis of apoptotic neutrophils, an essential process for the resolution of inflammation. We confirmed that 15d-PGJ2-treated Ams showed a significantly increased phagocytosis of apoptotic neutrophils by in vitro phagocytosis assay. The data indicate that PPARγ in Ams play an anti-inflammatory role through inhibiting cytokine production and increasing their CD36 expression with the enhancement of the capacity to phagocytose apoptotic neulrophils. Less
(1)25-羟维生素D_(31)α-羟化酶基因在人肺泡巨噬细胞中的表达我们用逆转录聚合酶链反应(RT-PCR)方法证实了25-羟维生素D_(31)α-羟化酶(1 αOHse)基因在人肺泡巨噬细胞(AM)中的表达。用半定量RT-PCR法测定的结节病患者的lα Hse-mRNA水平比其他肺部疾病的对照患者高5倍。支气管肺泡灌洗液中lα Ohse-mRNA水平与肺泡淋巴细胞相对数量、CD 4/CD 8比值、血清ACE、血清离子钙和lα,25-二羟化酶D3/25-羟维生素D3有良好的相关性(25 D3/1,25 D3)比值,提示AM中lα Ohse是功能性的,与结节病活动性及钙代谢密切相关。结果发现,肺癌组织中lα Ohse-mRNA的表达与肺癌的发生、发展密切相关, ...更多信息 明显增强,大约是对照组的两倍。1 α Ohse-mRNA表达水平与25 D3/I、25 D3比值呈正相关。AM中lα Ohse-mRNA的表达水平在晚期患者中有升高的趋势,但无统计学意义。(2)人肺泡巨噬细胞过氧化物酶体激活受体T(PeroxisomeCatalystator-Activated Receptor,PPART)的表达及功能研究PPARα、β、γ均表达,但以γ为主。15-脱氧-D12,14-前列腺素J2(15 d-PGJ 2)是PPARγ的一种特异性配体,可显著降低LPS诱导的Ams产生TNF-α,并显著增加介导凋亡中性粒细胞吞噬作用的CD 36的表达,这是炎症消退的一个重要过程。我们证实,15 d-PGJ 2处理的Ams显示出显着增加的吞噬凋亡中性粒细胞的体外吞噬试验。结果表明,Ams中的PPARγ通过抑制细胞因子的产生,增加CD 36的表达,增强吞噬凋亡中性粒细胞的能力而发挥抗炎作用。少

项目成果

期刊论文数量(24)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Inui N., Murayama A., Sasaki S., Chida K., Kato S., Nakamura H.: "Correlation between 25-hydroxyvitamin D3 l alpha-hydroxylase gene expression in alveolar macrophages and activity of sarcoidosis"Am J Med. 110・9. 687-693 (2001)
Inui N.、Murayama A.、Sasaki S.、Chida K.、Kato S.、Nakamura H.:“肺泡巨噬细胞中 25-羟基维生素 D31 α-羟化酶基因表达与结节病活性之间的相关性”Am J Med 110。・9。687-693(2001)
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Inui N., Murayama A., Sasaki S., Chida K., Kato S., Nakamura H.: "Correlation between 25-hydroxyvitamin D3 lalpha-hydroxylase gene expression in alveolar macrophages and activity of sarcoidosis"Am J Med. 110・9. 687-693 (2001)
Inui N.、Murayama A.、Sasaki S.、Chida K.、Kato S.、Nakamura H.:“肺泡巨噬细胞中 25-羟基维生素 D3 lα-羟化酶基因表达与结节病活性的相关性”Am J Med 110・9. 687-693 (2001)
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    0
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Xue H-H,Nakamura H, et al.: "Store depletion by caffeine/ryanodine activates capacitative ca2+ entry in nonexcitable A549 cells"J Biochem. 128(2). 329-336 (2000)
Xue H-H、Nakamura H 等人:“咖啡因/兰尼碱的储存消耗激活非兴奋性 A549 细胞中的电容性 ca2 进入”J Biochem。
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Sato J., Chida K., Suda T., Sato A., Nakamura H: "Migratory patterns of thoracic duct lymphocytes into bronchus-associated lymphoid tissue of immunized rats"Lung. 178・5. 295-308 (2001)
Sato J.、Chida K.、Suda T.、Sato A.、Nakamura H:“免疫大鼠胸导管淋巴细胞向支气管相关淋巴组织的迁移模式”178・5(2001)。
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Inui N., Murayama A., Sasaki S., Chida K., Kato S., Nakamura H.: "Correlation between 25-hydroxyvitamin D3 lalpha-hydroxylase gene expression in alveolar macrophages and activity of sarcoidosis"Am J Med. 110. 687-693 (2001)
Inui N.、Murayama A.、Sasaki S.、Chida K.、Kato S.、Nakamura H.:“肺泡巨噬细胞中 25-羟基维生素 D3 lα-羟化酶基因表达与结节病活性之间的相关性”Am J Med。
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NAKAMURA Hirotoshi其他文献

NAKAMURA Hirotoshi的其他文献

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{{ truncateString('NAKAMURA Hirotoshi', 18)}}的其他基金

Molecular mechanism of the type 2 deiodinase gene transcription
2型脱碘酶基因转录的分子机制
  • 批准号:
    20591092
  • 财政年份:
    2008
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
THE STUDY OF THE FUNCTIONS AND EXPRESSIONS OF WILD-TYPE AND MUTANT THYROID HORMONE RECEPTORS
野生型和突变型甲状腺激素受体功能和表达的研究
  • 批准号:
    08457262
  • 财政年份:
    1997
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Research on the Mechanism of thyroid hormone action
甲状腺激素作用机制的研究
  • 批准号:
    62570511
  • 财政年份:
    1987
  • 资助金额:
    $ 2.11万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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