Involvement of FSP1 positive fibroblasts in the progression of human glomerulonephntis

FSP1阳性成纤维细胞参与人肾小球肾炎的进展

• 批准号: 12671043
• 负责人: IWANO Masayuki
• 金额: $ 1.73万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671043/
• 关键词: glomerulonephritis; interstitial fibrosis; fibroblasts; FSP1; EMT; tubular epithelial cell; visceral epithelial cell; EMT

Localization of fibroblast specific protein 1 (FSP1) in kidney was studied by immunohistochemistry using anti-FSP1 polyclonal antibody. Renal biopsy specimens were obtained from 320 patients with various kinds of glomerulonephritis (IgA nephropathy, 142 patients ; membranous nephropathy, 28 patients; focal and segmental glomeruloscrelosis, 36 patients; minimal change nephritic syndrome, 31 patients ; lupus nephritis, 41 patients; crescentic glomerulonephritis 42 patients). FSP1 positive fibroblasts accumulated in the area showing severe interstitial fibrosis. Some tubular epithelial cells in the fibrosis area also expressed FSP1. The number of FSP1 positive cells was positively correlated with the area of fibrosis, which was measured using standard point-counting method (r = 0.84, p < 0.0001). The number of FSP1 positive cells was positively correlated with serum creatinine (r = 0.67, p < 0.0001) and negatively correlated with creatinine clearance (r = -0.71, p < 0.0001).We also examined glomerular FSP1 expression. In patients with crescentic glomerulonephritis, FSP1 was localized in visceral epithelial cells and in crescents. Levels of FSP1 expression in visceral epithelial cells in patients with focal and segmental glomeruloscrelosis was significantly higher than those in patients with minimal change nephritic syndrome.

应用免疫组织化学方法研究了成纤维细胞特异性蛋白1（FSP 1）在肾组织中的定位。对320例不同类型的肾小球肾炎（伊加肾病142例，膜性肾病28例，局灶节段性肾小球硬化36例，微小病变型肾炎31例，狼疮性肾炎41例，新月体肾炎42例）进行肾活检。FSP 1阳性成纤维细胞聚集在显示严重间质纤维化的区域。纤维化区部分肾小管上皮细胞也表达FSP 1。FSP 1阳性细胞数与纤维化面积呈正相关（r = 0.84，p < 0.0001）。FSP 1阳性细胞数与血清肌酐呈正相关（r = 0.67，p < 0.0001），与肌酐清除率呈负相关（r =-0.71，p < 0.0001）。在新月体肾炎患者中，FSP 1定位于内脏上皮细胞和新月体中。局灶性和节段性肾小球硬化患者内脏上皮细胞FSP 1表达水平显著高于微小病变型肾病综合征患者。