What kind of food protein remarkably enhances GFR in man

Hostetter曾报道，健康人摄入200 g牛排会导致GFR升高约120%。但是，没有研究使用相同数量的植物蛋白。大多数研究表明，原料在蒸煮前的氨基酸组成及其分析方法和氨基酸浓度的指示是模糊的。我们在获得知情同意的相同六名年轻男性受试者中尝试在急性负荷相同量的不同蛋白质（牛排或烤脱脂大豆）的情况下测量GFR（Gin）。每名受试者分别接受饥饿、牛排或烤脱脂大豆，饥饿、牛排或烤脱脂大豆交替进食，间隔一周，牛排和烤脱脂大豆的蛋白质含量调整为64.5g。除色氨酸（NaOH水解）外，用HCl水解和HPLC分析氨基酸组成。以饥饿时的GFR为对照。纯菊糖由Fuji Pharmaceut.Co.提供。清除试验采用Wesson法。采用Jaffe速率法测定肌酐清除率（Ccr）。同时测定血浆胰高血糖素和尿c-GMP。通过Student检验检查统计学显著性。牛排和烤脱脂大豆阶段的GIN值高于饥饿阶段。牛排和烤脱脂大豆阶段之间差异不显著。与饥饿期相比，两个阶段的Ccr均显著升高。三组血浆胰高血糖素升高的方式相似。但尿c-GMP排泄量无明显增加。牛排和脱脂黄豆中丙氨酸、甘氨酸和精氨酸的总量均为13.5g。我们的研究表明，植物蛋白（大豆）可以提高GFR高达动物（牛肉）蛋白。

Hostetter has reported 200g of beefsteak ingestion in healthy human subjects caused about 120% elevation of GFR. But, there have been no studies using the same amount of vegetable protein. Almost study indicated the amino acid composition of raw materials before cooking and the analytical methods and the indication of the concentration of amino acids are obscure. We have attempted GFR (Gin) measurement under acute loading of the same amount of different protein (beefsteak or baked defatted soybean) in the same six young male subjects obtained informed consent. Each subject has been received starvation, ingestion of beefsteak or baked defatted soybean.Starvation, beefsteak ingestion or soybean ingestion alternatively with interval of one week.The amount of protein was adjusted 64.5g both in beefsteak and baked deffated soybean. The amino acid composition was analysed with HC1 hydrolysis and HPLC except tryptohan(NaOH hydrolysis). GFR in the starvation was considered as control. Pure inulin was supplied by Fuji Pharmaceut.Co. Clearance test was performed by Wessons method. Creatinine clearance (Ccr) was determined by Jaffe rate assay method. Plasma glucagon and urinary c-GMP were also measured. Statistical significance was checked by Student test. Gin of beefsteak and those of baked defatted soybean stages elevated compared to that of starvation stage. No significant difference was observed among beefsteak and baked deffated soybean stage. Ccr elevated in both stages compared to starvation stage more remarkably. Plasma glucagon elevated a similar manner in three groups. But no significant increase in urinary excretion of c-GMP. Total amount of alanine, glycine and arginine was about 13.5g both in beefsteak and baked defatted soybean. Our study revealed that vegetable protein (soybean) could elevates GFR as high as animal (beef) protein.