The experimental study about the sensitivity of flow stimulation in hepatic sinusoidal cell and hepatocyte during simple cold storage in rat liver

大鼠肝脏简单冷藏期间肝窦细胞和肝细胞血流刺激敏感性的实验研究

• 批准号: 12671188
• 负责人: HIKIDA Shigeki
• 金额: $ 0.9万
• 依托单位: KURUME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671188/
• 关键词: liver transplantation; ischemia & reperfusion injury; flow stimulation; sinusoidal cell; hepatocyte; endothelin; Calcium antagonist; 冷保存; 肝臓移植; 虚血・再灌流; 微小循環; ずり応力

We investigated the the sensitivity of flow stimulation in sinusoidal cell and hepatocyte during simple cold storage in rat liver. In the preservation study, the liver was in simple cold storage for 6 hours. The zonal difference of morphological change was evaluated and this demonstrated that sinusoidal narrowing occurred significantly serious in zone 3, whose flow is the fastest of three zones, compared to zone 1 or zone2.In the reperfusion study, the zonal difference of hepatic sinusoidal blood flow was evaluated in 7 groups as follows : Group1; hepatic artery ligation as control, Group 2; Syngenic orthotopic liver transplantation without arterial reconstruction (OLTx) after Ohour simple cold preservation, Group3; OLTx after 6hr cold preservation, Group4; OLTx after 6hr cold preservation with intraportal flush at physiological portal pressure level just before transplantation, Group5; OLTx after 6hr cold preservation with intraportal flush at twice level of physiological portal pressure just before transplantation, Group 6; OLTx after 6hr cold preservation whose solution includes endothelin blocker, Group 7; OLTx after 6hr cold preservation whose solution includes calcium antagonist. The hepatic sinusoidal flow in the group 5 was smilar to that in the group1 in every zone. The flows of zone 2 and zone 3 in the group4, in the group 6, and in the group 7 were similar to that in the group1, however, the flows of zone1 in these 3 groups did not reach the group I level. The tissue malondialdehyde level in the group 5, in the group 6, and in the group 7 were significantly lower than those in the group 2,in the group 3, and in the group4. These results suggest that hepatic sinusoidal cell might have sensitivity for flow stimulation and the intraportal flush as physical force to dilate the hepatic sinusoid might be beneficial to reduce sinusoidal microcirculation disturbance.

本文研究了大鼠肝脏简单冷藏过程中，肝窦细胞和肝细胞对血流刺激的敏感性。在保存研究中，肝脏在简单冷藏中保存6小时。在再灌注研究中，比较了7组肝窦血流的差异：1组为对照组，单纯肝动脉结扎后不重建肝动脉(OLTx)组，3组冷保存6小时后的OLTx组，4组冷保存6小时后的OLTx组，5组冷保存6小时后冷保存6小时的OLTx组，5组。6组：冷保存6h后的OLTx，移植前门脉压力为生理门脉压力的2倍；7组，冷保存6h后的OLTx，其溶液中含有内皮素阻滞剂；5组的肝窦血流在各区段均与1组相似。4组、6组、7组的2区、3区血流与1组相似，而1区血流未达到I组水平。5组、6组、7组组织丙二醛水平明显低于2组、3组、4组。这些结果提示肝窦细胞对血流刺激可能具有敏感性，门静脉内冲洗作为扩张肝窦的物理力量可能有利于减少肝窦微循环障碍。