Specific CTL Activity of Lamina Propria Lymphocytes in Idiopathic Inflammatory Bowel Diseases.

特发性炎症性肠病中固有层淋巴细胞的特异性 CTL 活性。

• 批准号: 12671217
• 负责人: NITTA Toyoo
• 金额: $ 2.05万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671217/
• 关键词: idiopathic inflammatory bowel disease; 2,4,6-Trinitrobenzene-sulfonic acid(TNBS) induced colitis; cytotoxicity; TCR; V β repertoire

In this study, We analyzed the functional role of CD8^+TCRV β 14T-cells in 2,4,6-Trinitrobenzene-sulfonic acid(TNBS) induced colitis as follows ;1. ^<51>Cr release assay : CD8^+TCR V β 14 T-cell clones showed specific cytotoxic activity against TNBS conjugated self spleen cells.2. ELISA : These clones produced IFN-γ in culture supernatant, but neither IL-2 nor IL-4.3. Adoptive transfer : Recipient animals transferred with these clones developed colitis when exposed to low doses of TNBS.

在本研究中，我们分析了CD 8 ^+TCRV β 14 T细胞在2，4，6-三硝基苯磺酸（TNBS）诱导的结肠炎中的功能作用如下：1. Cr<51>释放试验：CD 8 +TCR V β 14 T细胞克隆对TNBS缀合的自身脾细胞显示特异性细胞毒活性。ELISA：这些克隆在培养上清液中产生IFN-γ，但不产生IL-2和IL-4。连续转移：当暴露于低剂量TNBS时，用这些克隆转移的哺乳动物发展结肠炎。