Specific CTL Activity of Lamina Propria Lymphocytes in Idiopathic Inflammatory Bowel Diseases.

在本研究中，我们分析了CD8^+TCRV β 14t细胞在2,4,6-三硝基苯磺酸（TNBS）诱导的结肠炎中的功能作用：^<51 bb0 Cr释放试验：CD8^+TCR V β 14 t细胞克隆对TNBS偶联的自身脾细胞具有特异性的细胞毒活性。ELISA：这些克隆在培养上清中产生IFN-γ，但不产生IL-2和IL-4.3。过继性转移：用这些克隆转移的受体动物在暴露于低剂量TNBS时发生结肠炎。

In this study, We analyzed the functional role of CD8^+TCRV β 14T-cells in 2,4,6-Trinitrobenzene-sulfonic acid(TNBS) induced colitis as follows ;1. ^<51>Cr release assay : CD8^+TCR V β 14 T-cell clones showed specific cytotoxic activity against TNBS conjugated self spleen cells.2. ELISA : These clones produced IFN-γ in culture supernatant, but neither IL-2 nor IL-4.3. Adoptive transfer : Recipient animals transferred with these clones developed colitis when exposed to low doses of TNBS.