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Basic Study on Preservation Conditions Of Donor's Lungs In Lung Transplantation - Development of The Standard Method for Evaluating Alveolar Type II Cells With Their Surfactant Synthetic Activities As An Indicator -

肺移植中供肺保存条件的基础研究 - 以表面活性剂合成活性为指标评价肺泡II型细胞标准方法的开发 -

基本信息

批准号：
12671296
负责人：
SUWABE Akira
金额：
$ 2.05万
依托单位：
IWATE MEDICAL UNIVERSITY (2001)Yamagata University (2000)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671296/
关键词：
LUNG TRANSPLANTATION ALVEOLAR TYPE II CELL SURFACTANT

项目摘要

Lung transplantation needs to be performed so that many of lung cell functions are well preserved. This time limit has been around 6 hours so far. Unfortunately, most of the patients has not been saved because of this time limit. If new preservation fluids or better preservation conditions are developed, lung transplantation will be opened for more patients. In this study, we intended to develop the standard methods for evaluating new preservation fluids or better preservation conditions using rat isolated alveolar type II cells.Type II cells were isolated from rat lungs using a standard method described by Mason, et el. Isolated type II cells were incubated for up to 20 hrs with ^3H choline, a precursor of surfactant lipid both in the standard medium (D-MEM) and in two 'kinds of preservation fluids; extracellular type Perfodex (P) solution and intracellular type Euro-Collins (EC) solution. Surfactant synthetic activity (uptake of ^3H-choline to ^3H-phoshatidylcholine) was greater in type II cells in P solution than in those in D-MEM and EC solutions.Next, we isolated type II cells from rat lungs which were perfused with P and EC solutions and preserved in the same solutions for 6 hours at 4 degree. After isolation, surfactant synthetic activities were estimated with the same methods. The number of type II cells isolated from rat lungs preserved in P solution was greater than that in EC solution. However, surfactant synthetic activity was similar in both type II cells.In conclusion, the extracellular type P solution was suitable in lung preservation in a view of surfactant synthetic activity as a functional marker of type II cells.

需要进行肺移植，以便很好地保留许多肺细胞功能。到目前为止，这一时限约为6小时。不幸的是，大多数病人都没有因为这个时间限制而得救。如果开发出新的保存液或更好的保存条件，肺移植将为更多的患者开放。在这项研究中，我们打算开发标准方法，以评价新的保存液或更好的保存条件，使用大鼠肺泡II型细胞分离。将分离的II型细胞与^3H胆碱（表面活性剂脂质的前体）一起在标准培养基（D-MEM）和两种保存液（细胞外型Perfodex（P）溶液和细胞内型Euro-Collins（EC）溶液）中孵育20小时。Ⅱ型细胞在P溶液中的表面活性物质合成活性（将^3H-胆碱摄取为^3H-磷脂酰胆碱）高于在D-MEM和EC溶液中的活性物质合成活性。分离后，用相同的方法估计表面活性剂合成活性。从P液保存的大鼠肺组织中分离的II型细胞数量多于EC液。但两种II型细胞的表面活性物质合成活性相似。总之，鉴于表面活性物质合成活性可作为II型细胞的功能标志，细胞外P型液适用于肺保存。