Basic Study on Preservation Conditions Of Donor's Lungs In Lung Transplantation - Development of The Standard Method for Evaluating Alveolar Type II Cells With Their Surfactant Synthetic Activities As An Indicator -

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Lung transplantation needs to be performed so that many of lung cell functions are well preserved. This time limit has been around 6 hours so far. Unfortunately, most of the patients has not been saved because of this time limit. If new preservation fluids or better preservation conditions are developed, lung transplantation will be opened for more patients. In this study, we intended to develop the standard methods for evaluating new preservation fluids or better preservation conditions using rat isolated alveolar type II cells.Type II cells were isolated from rat lungs using a standard method described by Mason, et el. Isolated type II cells were incubated for up to 20 hrs with ^3H choline, a precursor of surfactant lipid both in the standard medium (D-MEM) and in two 'kinds of preservation fluids; extracellular type Perfodex (P) solution and intracellular type Euro-Collins (EC) solution. Surfactant synthetic activity (uptake of ^3H-choline to ^3H-phoshatidylcholine) was greater in type II cells in P solution than in those in D-MEM and EC solutions.Next, we isolated type II cells from rat lungs which were perfused with P and EC solutions and preserved in the same solutions for 6 hours at 4 degree. After isolation, surfactant synthetic activities were estimated with the same methods. The number of type II cells isolated from rat lungs preserved in P solution was greater than that in EC solution. However, surfactant synthetic activity was similar in both type II cells.In conclusion, the extracellular type P solution was suitable in lung preservation in a view of surfactant synthetic activity as a functional marker of type II cells.