Functional modulation and control of osteoclastgenesis by wear debris and inflammatory cytokines.

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I. To clarify the role of titanium particles on osteoblast viability and cell proliferation, two human osteoblastic cells (MG-63 and SaOS2) and human osteoblasts were cultured in the presence or absence of titanium particles. The viability and cell proliferation were deteriolated as concentrations of the partikles increased. These results suggest that titanium particles may reduce the bone formation arround implants.II. To clarify the role of titanium particles on osteoblast MMPs-TIMPs system, quantitative PCR analysis was performed. In early time exposure, MMP-1 production was increased, and after long time exposure, MMP-1, -2, and TIMP-1, -2 production in ribonucleic acid levels were suppressed.This data suggested that titanium particles between bone and implants may provide the space of osteoclast attachment on bone surface to enhance osteoclastic bone resorption.These results suggest that titanium particles generated between bone and implants may play an important role for bone resorption through 1) reduction of bone formation and 2) accerelate osteoclastic bone resorption.