Gene TheraphyforSpinal Cord Injury

高压氧治疗对脊髓损伤急性期诱导型一氧化氮合酶(INO)和胶质细胞源性神经营养因子(GDNF)的影响。为探讨高压氧对脊髓损伤的急性作用，采用逆转录-聚合酶链式反应(RT-PCR)检测脊髓损伤后GDNFmRNA和iNOSmRNA的表达。高压氧在脊髓损伤后30minitsu内有效，HBO在30minitsu后有效，而不是在伤后30minitsu；大鼠急性脊髓挫伤后，胶质细胞源性神经营养因子(GDNF)表达上调。采用重量下降技术，通过逆转录聚合酶链式反应(RT-PCR)检测大鼠急性脊髓挫伤后GDNF基因表达的时空变化，发现GDNF在脊髓损伤后30min开始表达上调，3h达到高峰。免疫组织化学分析显示，伤后1d，GDNF免疫反应主要出现在小胶质细胞和顶体细胞中，而神经元和星形胶质细胞中未见表达。这种GDNF基因表达的即刻上调可能是炎症过程的一个组成部分，并可能对脊髓损伤(SCI)后的神经元产生保护作用。

Elect of hyperbaric oxygen therapy on iNOand GDNF in the acute phase of spinal cord injury.To study the acute effect of hyperbaric oxygen ventilation (HBO) on spinal cord injury.We examined GDNFmRNA and iNOSmRNA using Reverse transcriptase-polymerase chain reaction (RT-PCR).HBO was effective within 30minitsu after spinal cord injury but the HBO after 30minitsu, instead of the times, was not effective.Up-regulation of glial cell line-derived neurotrophic factor(GDNF) following traumatic spinal cord injury.We investigated the temporal and spatial expression patterns of the GDNF gene after subjecting rats to an acute contusion injury of the spinal cord using the weight drop technique Reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that GDNF transcription in the spinal cord began to increase within 30 min after injury and peaked within 3h. Immunohistochemical analysis showed GDNF immunoreactivity to be present mainly in microglia and acrophages 1 day after injury, but not in the neurons or astrocytes. This immediate upreguration of GDNF gene expression may be a component of an inflammatory process and probably exerts aprotective effect on neurons following apinal cord injury(SCI).