A nove gene therapy for Pseudomonas aeruginosa pneumonia.

1、的初步实验经呼吸道滴注产生外显素和外显素缺陷的铜绿假单胞菌PA103等基因突变株(PA103DUT)不会引起肺损伤，在肺内可见可有效预防肺损伤。2、假单胞菌肺炎的动物模型我们在大鼠模型上评价了宿主对铜绿假单胞菌肺炎和伴发败血症的反应。3腺病毒载体、的构建我们已经构建了表达重组大鼠IL-10基因的腺病毒载体。我们还构建了表达重组大鼠角质形成细胞生长因子(KGF)基因的腺病毒载体。KGF是肺泡II型细胞的有效生长因子，有望预防急性肺损伤所致的肺损伤。我们在体内外证实了KGF在体内外的表达和表达。我们将在急性肺损伤模型中测试该载体的效果。

1、Preliminary experimentsThe airway instillation of isogenic mutant of Pseudomonas aeruginosa strain PA103 that is defective for production of ExoU and ExoT (PA103DUT) was shown not to cause lung injury, which was seen in the lung would be effective in preventing lung injury.2、An animal model for Pseudomonal pneumoniaWe evaluated the host response to Pseudomonas aeruginosa pneumonia and concomitant sepsis in a rat model. A manuscript is accepted for publication3、Construction of adenovirus vectorsWe have made adenovirus vector expressing the recombinant rat IL-10 gene. We also constructed adenovirus vector expressing the recombinant rat keratinocyte growth factor (KGF) gene. KGF is expected to prevent lung injury in acute lung injury for KGF is a potent growth factor for alveolar type II cells. We have confirmed the expression and production of KGF with the infection of this vector in vitro and in vivo. We are going to test the effect of the vector in an acute lung injury model.