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Effect of Heart Shock Protein and Interferon on Toxoplasmic Chorioretinitis

心休克蛋白与干扰素对弓形虫性脉络膜视网膜炎的影响

基本信息

批准号：
12671697
负责人：
NOROSE Kazumi
金额：
$ 2.11万
依托单位：
CHIBA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671697/
关键词：
Toxoplasma chorioretinitis heat shock protein interferon gamma organ specificity interferon gamma knock out mouse

项目摘要

1. To determine the influence of interferon (IFN)-γ on the organ infectivity, on the Toxoplasmic chorioretinitis, and on the genetic susceptibility of susceptible (C57BL/6) and resistant (BALB/c) strains after peroral infection with cystis of Toxoplasma gondii. IFN-γ knockout (KO) mice in C57BL/6 and BALB/c backbrounds were utilized. The kinetics of the changes in T.gondii abundance were evaluated with a quantitative competitive polymerase chain reaction assay in various organs including eyes at different times after peroral infection. In IFN-γ KO mice, a T.gondii-specific gene, SAG1, was detected in all organs examined, and the protozoan proliferated much more actively than in wild-type mice. The abundance of T.gondii was much higher in mesenteric lymph nodes and the heart than in other organs. In contrast, in the nervous system organs and kidneys, only a weakly detectable reaction was observed. Toxoplasma gondii grew at a more rapid rate in the organs of IFN-γ KO C57BL/6 mice than in the organs of IFN-γ KO BALB/c mice during the course of infection. Destruction of the INF-γ showed remarkable effects on the infectivity in both susceptible and resistant mice.2. The high-level expression of gondii HSP70 was correlated with mortality in IFN-γ KO mice infected with T.gondii.3. SAG1 was detected in the eye of IFN-γ KO mice infected with T.gondii perollary.

1.目的探讨γ-干扰素（IFN-γ）对弓形虫囊体经口感染后的器官感染性、弓形虫性脉络膜视网膜炎的影响以及对敏感株（C57 BL/6）和耐药株（BALB/c）的遗传易感性的影响。使用C57 BL/6和BALB/c背景中的IFN-γ敲除（KO）小鼠。采用竞争性定量聚合酶链反应（PCR-PCR）技术，对弓形虫经口感染后不同时间内眼等器官弓形虫数量的动态变化进行了研究。在IFN-γ KO小鼠中，在检查的所有器官中检测到刚地弓形虫特异性基因SAG 1，并且原生动物的增殖比野生型小鼠活跃得多。弓形虫在肠系膜淋巴结和心脏中的丰度明显高于其他器官。相反，在神经系统器官和肾脏中，仅观察到微弱的可检测反应。在感染过程中，弓形虫在IFN-γ KO C57 BL/6小鼠的器官中的生长速度比在IFN-γ KO BALB/c小鼠的器官中的生长速度快。INF-γ的破坏对敏感和抗性小鼠的感染性均有显著影响.弓形虫HSP 70的高表达与IFN-γ KO小鼠感染弓形虫后的死亡率相关.在IFN-γ基因敲除小鼠感染弓形虫后，眼内可检测到SAG 1。