Effect of Heart Shock Protein and Interferon on Toxoplasmic Chorioretinitis

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1. To determine the influence of interferon (IFN)-γ on the organ infectivity, on the Toxoplasmic chorioretinitis, and on the genetic susceptibility of susceptible (C57BL/6) and resistant (BALB/c) strains after peroral infection with cystis of Toxoplasma gondii. IFN-γ knockout (KO) mice in C57BL/6 and BALB/c backbrounds were utilized. The kinetics of the changes in T.gondii abundance were evaluated with a quantitative competitive polymerase chain reaction assay in various organs including eyes at different times after peroral infection. In IFN-γ KO mice, a T.gondii-specific gene, SAG1, was detected in all organs examined, and the protozoan proliferated much more actively than in wild-type mice. The abundance of T.gondii was much higher in mesenteric lymph nodes and the heart than in other organs. In contrast, in the nervous system organs and kidneys, only a weakly detectable reaction was observed. Toxoplasma gondii grew at a more rapid rate in the organs of IFN-γ KO C57BL/6 mice than in the organs of IFN-γ KO BALB/c mice during the course of infection. Destruction of the INF-γ showed remarkable effects on the infectivity in both susceptible and resistant mice.2. The high-level expression of gondii HSP70 was correlated with mortality in IFN-γ KO mice infected with T.gondii.3. SAG1 was detected in the eye of IFN-γ KO mice infected with T.gondii perollary.