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Organ Cell Biology of Salivary Secretion: Integrated analyses by combined approaches

唾液分泌的器官细胞生物学：组合方法的综合分析

基本信息

批准号：
12671781
负责人：
SEGAWA Akihisa
金额：
$ 2.18万
依托单位：
Kitasato University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671781/
关键词：
Salivary gland Secretion Calcium signaling Gap junction Tissue architecture Cell biology 甲状腺侏儒症

项目摘要

An organ is a mass of diverse cells possessing various morphological and functional identities, yet it can behave as an integrated unity. This study was aimed to clarify the structural bases that ensure organs to acquire the harmonized function. Salivary gland function is primarily achieved by the compartmentalized epithelial domains, acini and ducts, which also exhibit characteristic morphological specialization. High-speed confocal microscopy showed distinct pattern of calcium signaling between acini and ducts. Cells in acini responded synchronously while those in ducts responded asynchronously. A gap junction molecule, connexin 32, was immunolocalized predominantly between the acinar cells; thus the calcium signaling system in salivary glands is constructed specifically according to the tissue architecture. Beta-galactosidase knock-out mice revealed the appearance of vacuolar structures in the acinar domain and the granulated duct domain. A rat with hypothyroydism, rdw, exhibited the lack of the granulated duct segment. Beta-galactosidase and thyroxine are considered to play significant roles in the morphogenesis of acinar and duct segments. I was concluded that the segment specific differentiation of epithelia plays fundamental roles in controlling the harmonized organ function.

一个器官是一个具有不同形态和功能特性的细胞群，但它可以作为一个完整的整体。本研究旨在阐明器官获得协调功能的结构基础。唾液腺的功能主要是通过上皮细胞的区室结构、腺泡和导管来实现的，它们也表现出特征性的形态特化。高速共聚焦显微镜显示腺泡和导管之间钙信号的不同模式。腺泡中的细胞同步响应，而导管中的细胞异步响应。间隙连接分子，连接蛋白32，主要是免疫定位之间的腺泡细胞，因此在唾液腺中的钙信号系统是专门根据组织结构构建。β-半乳糖苷酶基因敲除小鼠的腺泡结构域和颗粒状导管结构域的空泡结构的外观。甲状腺功能减退大鼠rdw表现为颗粒管节段缺失。β-半乳糖苷酶和甲状腺素被认为在腺泡和导管段的形态发生中起重要作用。结论：上皮细胞的节段特异性分化在协调器官功能中起着重要作用。