Search for the expression of estrogenic action of dental materials

寻找牙科材料雌激素作用的表达

• 批准号: 12671867
• 负责人: NEGISHI Hideyuki
• 金额: $ 1.79万
• 依托单位: Kanagawa Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671867/
• 关键词: Dental Materials; Endocrine Disruptors; Estrogenic Activity; MCF-7; Bisphenol A; Sealant; Polycarbonate

Recent studies have been saised about the possibility that one of estrogenic chemicals, bisphenol A (BPA) might be leached out of dental sealants. This study aimed to identify and quantify BPA and other components released from sealants and dental polycarbonate materials in vitro. The sealants tested were not shown to have released BPA analyzed by a liquid chromatography. The three dental polycarbonate materials contained the amounts of 28.8 to 203 μg of BPA pre gram of pellet. But the content of BPA of dental PC material extracted with PBS(-) was less than μg level per gram dental PC material.The estrogen-sensitive MCF-7 breast canser cells was a useful tool that may estimate an estrogenic chemicals (environmental estrogens, xenoestrogens). When incubated with MCF-7 cultured with dextran-charcoal treated FCS and phenol red-free medium, BPA, 17 β-estradiol, diethylstilbestrol (DES) and ethynylestradiol promoted cell proliferation that was assayed the DNA content by DABA method, and induced the expression of progesterone receptor mRNA that was analyzed by RT-PCR. The extracts of dental sealants and dental PC materials with PBD(-) were not affected the proliferation and the expression of progesterone receptor of MCF-7.

最近的研究表明，一种雌激素化学物质双酚A （BPA）可能会从牙科密封剂中滤出。本研究旨在鉴定和定量体外密封胶和牙科聚碳酸酯材料中释放的双酚a和其他成分。经液相色谱分析，被测试的密封剂没有释放BPA。三种牙科用聚碳酸酯材料的BPA含量为28.8 ~ 203 μg /克。而PBS（-）提取的牙科PC材料中BPA含量均低于μg / g。雌激素敏感的MCF-7乳腺癌细胞是一个有用的工具，可以估计雌激素化学物质（环境雌激素，异种雌激素）。用葡聚糖-炭处理的FCS和无酚红培养基培养MCF-7，双酚a、17 β-雌二醇、己烯雌酚（DES）和乙炔雌酚促进细胞增殖，采用DABA法测定DNA含量，并诱导孕酮受体mRNA的表达，采用RT-PCR分析。含PBD（-）的牙科密封剂和牙科PC材料提取物对MCF-7的增殖和孕酮受体的表达没有影响。

项目成果

Saito,M., Iwase,M., Maslan,S., Nozaki,M., Yamauchi,M., Handa,K., Takahashi,O., Sato,S., Kawase,T., Teranaka,T., Narayanan,A.S.: "Expression of Cementum-derived Attachment Protein in Bovine Tooth Germ During Cementogenesis"Bone. 29. 242-248 (2001)

斋藤，M.，岩濑，M.，马斯兰，S.，野崎，M.，山内，M.，半田，K.，高桥，O.，佐藤，S.，川濑，T.，寺中，T.，

室井剛史: "歯科用ポリカーボネート中のビスフェノールAの定量と溶出動態について"神奈川歯学. 37. 76-82 (2002)

Takeshi Muroi：“牙科聚碳酸酯中双酚 A 的定量和溶解动力学”《神奈川牙科科学》37. 76-82 (2002)。

室井剛史: "歯科用ポリカーボネート中のビスフェノールAの定量と溶出動態について"神奈川歯科大学学会雑誌. 第37巻第2,3号. 76-82 (2002)

Takeshi Muroi：“牙科用聚碳酸酯中双酚 A 的定量和溶解动力学”神奈川齿科大学学报，第 37 卷，第 2 期和第 3 期。76-82（2002 年）。

藤條一江: "ヒト歯槽骨由来骨芽細胞様細胞におけるbFGFの効果"小児歯科学雑誌. 38. 1119-1129 (2000)

Kazue Fujijo：“bFGF 对源自人牙槽骨的成骨细胞样细胞的影响”《儿科牙科杂志》38. 1119-1129 (2000)。

Kawase,T.: "Tissue Engineering and its Perspective for the Regeneration of periodontium"J of the Japanese Society of periodontology. 43-4. 347-352 (2001)

Kawase,T.：“组织工程及其对牙周组织再生的展望”，日本牙周病学会杂志。