Analysis of abnormal DNA structure and down regulation of a new tumor suppressor, FEZ1 gene, in oral squamous cell carcinoma.

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Allelic deletions on the short arm of chromosome 8 (8p) are frequent events inseveral human malignancies, including oral cancer. We have examined and found two common regions of deletion on 8p (8pl2, 8p22) in oral squamous cell carcinomas (SCC) s. The possible involvement of FEZl/LZTSl (FEZ1) gene, a candidate tumor suppressor gene, mapped at 8p22, was also evaluated. Here we analyzed whether FEZl alterations play arole in the development and progression of oral SCCs. In present study, we examined FEZ1 expression in 31 primary oral SCCs and 8 SCC-derived cell line by reverse transcription-PCR (RT-PCR). Thirty-five % of tumor (ll of 31) and 100% of cell lines (8 of 8) showed absent or reduced mRNA gene expression. Furthermore, to investigate the mechanism for silencing, cell were cultured with 5-aza-2'-deoxycytidine and all of cell linesshowed restoration by demethylating agent. These findings suggest that inactivation of the FEZ1 gene may contribute to the development of oral SCCs.