Study for enhancement of VEGF production and angiogenesis in human Human oral squamous cell carcinoma cell

血管生成是实体瘤生长和转移的重要现象。本研究考察了人类口腔鳞状细胞癌细胞系产生的血管生成因子的特征。转移能力最强的HSC3条件培养液可产生大量的血管内皮细胞生长因子，促进内皮细胞的管状形成。促进HSC-3血管生成的机制是1)高SP-1的结构性表达，2)诱导血管内皮生长因子受体的表达。细胞还产生透明质酸酶-1和低分子透明质酸，这是血管生成因子之一。HSC-3还产生色素上皮源性因子，即抗血管生成因子(PEDF)。PEDF的产生受血管内皮生长因子的调节，因此，血管内皮生长因子和PEDF之间的平衡调节

Angiogenesis is an important phenomenon for the growth and metastasis of solid tumors. The present study examined the characterization of angiogenic factors produced by human oral squamous cell carcinoma (oral SCC) cell lines. The conditioned medium of HSC3 with the strongest metastatic ability among the examined lines produced high amount of VEGF and enhanced tube formation of endothelial cells. The mechanisms for enhancing angiogenesis in HSC-3 is due to 1)constitutive expression of high SP-1, 2)production of the factors inducing expression of VEGF receptor. The cells also produced hyaluronidase-1 and produced low molecular hyaluronic acid, which is one of the angiogenic factor. HSC-3 also produced pigmental epithelium-derived factor, which is anti-angiogenic factor (PEDF). The production of PEDF is regulated by VEGF , and as a result the balance between VEGF and PEDF regulates