Studies on the inhibition to its biofilm formation by an antibody against the coaggregation of periodontopathic bacteria

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In this project, we analyzed the inhibition to its biofilm formation by an antibody against the coaggregation of periodontopathic bacteria. We have reported that our purified L-arginine sensitive hemagglutinin (A-HA) from Fusobacterium nucleatum ATCC10953 has a high potential hemagglutination activity. We have and characterized L-arginine sensitive hemagglutinin (A-HA) from F. necleatum ATCC10953, and reported the dominant role of A-HA in the adherence of the bacterium to streptococci.For the purpose of making a study of the effects of the inhibition to bacterial biofilm formation at periodontal pockets by the antibody as a target for A-HA, we examined a basic experimentation to the following effect.1. The A-HA rabbit polyclonal antibody inhibited not only the hemagglutination with F. nucleatum and human erythrocytes but also the coaggregation of F. nucleatum with Campybacter rectus, Porphyromonas gingivalis, Prevotella intermedia or Eikenella corrodens.2. The human IgG antibody were extracted from the serum of patients with periodontal disease inhibited the hemagglutination and the coaggregation in the same way as the case of the rabbit polyclonal antibody.3. A mouse monoclonal antibody against the A-HA was made from the hybridoma by fusion with mouse myeloid cell 31 and mouse Balb/c lymph node cell.4. The bacterial biofilm formation experiment (in vitro) which the way of co-culture of periodontopathic bacteria was used for was made to be established.It was became clear with the A-HA that it had the activity of the hemagglutination and the coaggregation. It was suggested that an anti-A-HA antibody was effective in the inhibition of the baterial biofilm formation at periodontal pockets, for the reason that the antibody inhibited the hemaglutination and the coaggregation.