Studies on the inhibition to its biofilm formation by an antibody against the coaggregation of periodontopathic bacteria

牙周病菌共聚抗体抑制其生物膜形成的研究

• 批准号: 12672035
• 负责人: KAWAMOTO Toru
• 金额: $ 2.18万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672035/
• 关键词: periodontopatic bacteria; coaggregation factor; antibody; Fusobacterium nucleatum; bacterial biofilm; hemagglutinin; fusobacterium nucleatum; 最近バイオフィルム; 赤血球凝集因子

In this project, we analyzed the inhibition to its biofilm formation by an antibody against the coaggregation of periodontopathic bacteria. We have reported that our purified L-arginine sensitive hemagglutinin (A-HA) from Fusobacterium nucleatum ATCC10953 has a high potential hemagglutination activity. We have and characterized L-arginine sensitive hemagglutinin (A-HA) from F. necleatum ATCC10953, and reported the dominant role of A-HA in the adherence of the bacterium to streptococci.For the purpose of making a study of the effects of the inhibition to bacterial biofilm formation at periodontal pockets by the antibody as a target for A-HA, we examined a basic experimentation to the following effect.1. The A-HA rabbit polyclonal antibody inhibited not only the hemagglutination with F. nucleatum and human erythrocytes but also the coaggregation of F. nucleatum with Campybacter rectus, Porphyromonas gingivalis, Prevotella intermedia or Eikenella corrodens.2. The human IgG antibody were extracted from the serum of patients with periodontal disease inhibited the hemagglutination and the coaggregation in the same way as the case of the rabbit polyclonal antibody.3. A mouse monoclonal antibody against the A-HA was made from the hybridoma by fusion with mouse myeloid cell 31 and mouse Balb/c lymph node cell.4. The bacterial biofilm formation experiment (in vitro) which the way of co-culture of periodontopathic bacteria was used for was made to be established.It was became clear with the A-HA that it had the activity of the hemagglutination and the coaggregation. It was suggested that an anti-A-HA antibody was effective in the inhibition of the baterial biofilm formation at periodontal pockets, for the reason that the antibody inhibited the hemaglutination and the coaggregation.

在这个项目中，我们分析了它的生物膜形成的抑制抗体对牙周致病菌的共聚集。本文报道了从具核梭杆菌ATCC 10953中分离纯化的L-精氨酸敏感血凝素（A-HA）具有较高的血凝活性。从F. necleatum ATCC 10953的抗体，并报道了A-HA在细菌对链球菌的粘附中的主导作用。为了研究A-HA作为靶点的抗体对牙周袋细菌生物膜形成的抑制作用，我们进行了以下效果的基础实验。A-HA兔多克隆抗体不仅能抑制F. nucleatum和人红细胞的共聚集性，以及F.具核弯曲杆菌、牙龈卟啉单胞菌、中间普雷沃菌或腐蚀艾肯菌。从牙周病患者血清中提取的人IgG抗体与兔多克隆抗体一样，对血凝和共聚集有抑制作用.将该杂交瘤与小鼠骨髓细胞31和小鼠Balb/c淋巴结细胞融合，制备了抗A-HA的单克隆抗体.采用牙周病细菌共培养法进行细菌生物膜形成实验（体外），结果表明A-HA具有血凝和共聚集活性。提示抗A-HA抗体能有效抑制牙周袋内细菌生物膜的形成，其原因是抗体能抑制血凝和共聚集。

项目成果

Munemasa T., ef al.: "Adeherence of Bacteroides forsythus to host cells"Microbios. 101. 115-126 (2000)

Munemasa T.等人：“福赛拟杆菌对宿主细胞的粘附”微生物。

Munemasa T.,Takemoto T. ほか: "Adherence of Bacteroides forsythus to host cells"Microbios. 101. 115-126 (2000)

Munemasa T.、Takemoto T. 等人：“福赛拟杆菌对宿主细胞的粘附”微生物学 101. 115-126 (2000)

Munemasa T: "Adeherence of Bacteroides forsythus to host cells"Microbios. 101. 115-126 (2000)

Munemasa T：“福赛拟杆菌对宿主细胞的粘附”微生物。

Munemasa, T., et al.: "Adeherence of Bacteroides forsythus to host cells"Microbios. 101. 115-126 (2000)

Munemasa, T., et al.：“福赛拟杆菌与宿主细胞的粘附”微生物。

Okada, M., et al.: "Clinical periodontal findings and microflora profiles in children with chronic neutropenia under supervised oral hygiene"J.Periodontol.. 72. 945-952 (2001)

Okada, M., 等人：“在口腔卫生监督下患有慢性中性粒细胞减少症的儿童的临床牙周表现和微生物群谱”J.Periodontol.. 72. 945-952 (2001)