Assessment of biological effect of low dose butyltin concentrations using small crustaceans with short-life span

在许多被认为具有内分泌干扰作用的人造化学品中，对三丁基锡（TBT）的严重生态毒理学影响的关注导致大多数发达国家对水产养殖和小船使用三丁基锡实行法律的限制。Caprella spp.是一种小型片足类甲壳动物，运动减少，寿命不到3个月。此外，Caprella spp.缺乏降解三丁基锡的能力。这些特征表明，Caprella spp.是对三丁基锡化合物最敏感的海洋无脊椎动物之一。2001年在濑户内海西部及邻近海域进行的丁基锡现场调查表明，丁基锡的TBT范围为N.D. (not检测到）降至39.1纳克三丁基锡化合物/升。为了评价三丁基锡化合物（TBT）对红球藻的生物学效应，在胚胎发育过程中暴露于0 ~ 10，000 ng TBTCl/L的5种浓度的丹氏Caprella danilevskii幼鱼，在无污染海水中饲养1代以上。即使在10 ng TBTCl/L时，成熟期的存活率也低于50%.因此，2001年在濑户内海检测到的三丁基锡化合物浓度仍然影响着Caprella spp的存活率。

Of many artificial chemicals which are though to have endocrine disruptor effects, the concern over the serious ecotoxicological impact of tribtyltin (TBT) led to legal restrictions on the usage of TBT in aquaculture and small boats in the majority of developed countries. Caprella spp.are small amphipod crustaceans with reduced movement and a life-span of less than 3 months. Moreover, Caprella spp.have lack the ability of degenerating TBT (tributyltin). These characteristics indicate that Caprella spp.are one of the most sensitive marine invertebrates to TBT. The field survey from the western part of Seto Inland Sea and adjacent areas on the butyltins during 2001 indicated that the TBT ranged N.D.(not detected) to 39.1 ng TBT/L. To assess the biological effect of TBT to Caprella., juveniles of Caprella danilevskii, which had been exposed to 5 concentrations from 0 to 10,000 ng TBTCl/L during embryonic development, reared in non-contaminated seawater more than 1 generation. The survival rate at the mature stage was less than 50 % even at 10 ng TBTCl/L. Thus, the TBT concentrations detected from the Seto Inland Sea during 2001 still influenced the survival rate of Caprella spp.