ANTIBIOTICS CIRCURATION AND BIOLOGICAL ACCUMURATION IN SEMIENCLOSED SEA

半封闭海中的抗生素循环和生物积累

• 批准号: 12680572
• 负责人: MUNEKAGE Yukihiro
• 金额: $ 2.18万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680572/
• 关键词: Antibiotics for fisheries; Biological accumulation; Antibiotic-resistant bacteria; Shrimp rearing in mangrove area; Degradation of antibiotics under UV; Mangrove development and environment destruction; 魚病; マングローブ開発と環境破壊; 高速液体クロマトグラフ(HPLC); 懸濁物質

The concentrations of two kinds of antibiotics (ABPC and OTC) in seawater and bottom mud were measured in the fisheries ground. Other four kinds of antibiotics (trimethoprim (TMP), sulfamethoxazole (SMX), norfoxacin (NFXC), oxolinic acid (OXLA)) were extracted from water and bottom mud in Vietnam shrimp rearing ponds developed in the mangrove area and their concentrations were measured. The degradation rate of antibiotics under UV and its absorption and accumulation in plankton were also measured.1) AMP and OTC concentration in water were fluctuated 0.56-1.4ppb and 0.25-4.9ppb, respectively.2) AMP and OTC concentration in bottom mud in fish culture area were fluctuated 15-25ug/1g dry mud and 0.15-0.35ug/1g dry mud, respectively. Accumulated AMP and OTC in 10cm mud depth in Uranouchi Bay were 8ton and 80kg, respectively.3) Antibiotics were degraded under UV. Its concentration C(t) was indicated by exponential function like as C(t)=C_iexp(-α t), where t is time, α is effected by initial … More concentration, C_i. Low concentration OTC is easy to be degraded, but difficult in high concentration of over lOmM, particularly AMP.4) Plankton grew in the 1/2 F medium with OTC, the higher the OTC concentration, the higher the growth rate of plankton and accumulation rate of OTC into plankton cells.5) Concentration of TMP in Vietnam shrimp pond water was 0.3〜1.1ppm in the surface water, 0.3〜2.1ppm in bottom water, Maximum SMX was 1.8〜5.6ppm, maximum NFXC was 1〜4ppm, OXLA was 0.5〜1.8ppm.6) TMP was accumulated at 0.02〜0.13mg, SMX was 0.01〜0.08mg, NFXC Was 0.1〜2.6mg, OXLA was 0.1mg in bottom wet mud of 1g in Vietnam shrimp pond. Antibiotics were terrible highly contained in water and accumulated in bottom mud of shrimp pond.7) Low degradation antibiotics were contained highly in the seawater and accumulated in the bottom mud. Antibiotics stimulated the growth of plankton, and antibiotics were accumulated in plankton. Antibiotic-resistant bacteria appear in these antibiotic contaminated environments, and shrimp diseases such as WSSV (White spot syndrome shrimp virus), YHSV (Yellow head shrimp virus) and TSSV (Taurin syndrome virus) breakout every year. Less

测定了渔场海水和底泥中两种抗生素(ABPC和OTC)的浓度。从越南红树林区对虾养殖池的水体和底泥中提取了甲氧苄啶(TMP)、磺胺甲恶唑(SMX)、诺福沙星(NFXC)和恶唑酸(OXLA)4种抗生素，并测定了它们的浓度。测定了抗生素在紫外光下的降解速率及其在浮游生物中的吸收和积累。1)水中AMP和OTC的浓度分别在0.56-1.4 ppb和0.25-4.9 ppb之间波动。2)养鱼区底泥中的AMP和OTC的浓度分别在15-25ug/1g干泥和0.15-0.35ug/1g干泥之间波动。乌拉努奇湾10 cm泥层中AMP和OTC的积累量分别为8吨和80 kg。3)抗生素在紫外光下降解。其浓度C(T)由指数函数C(T)=C_iexp(-αt)表示，其中t为时间，α受初始…的影响4)浮游生物在含有OTC的1/2 F培养基中生长，OTC浓度越高，浮游生物的生长速率和向浮游生物细胞中积累OTC的速率越高。5)越南虾池水中TMP的浓度表层为0.3~1.1ppm，底层为0.3~2.1ppm，最大SMX为1.8~5.6ppm，最大NFXC为1~4ppm。6)越南虾池底泥中TMP积累量为0.02~0.13 mg，SMX为0.01~0.08 mg，NFXC为0.1~2.6 mg，OXLA为0.1 mg。7)低降解抗生素在海水中含量较高，在底泥中积累。抗生素刺激浮游生物的生长，并在浮游生物中积累抗生素。在这些抗生素污染的环境中出现了抗药性细菌，每年都会爆发WSSV(白斑综合征对虾病毒)、YHSV(黄头虾病毒)和TSSV(牛磺酸综合征病毒)等对虾疾病。较少

项目成果

宗景志浩 他: "沿岸海水中の微量化学物質(ABPC,OTC)の分布と消失に関する研究"海岸工学論文集. 48(未定). (2001)

Shihiro Munekage 等人：“沿海海水中微量化学物质（ABPC、OTC）的分布和消失研究”沿海工程杂志 48（待定）。

Yukihiro Munekage, Nozomu Iwasaki, Tuan Xuan Le, Hiroshi Hasegawa, Yuki Natukawa, Sakiko Kikuhara: "Studies on the Distribution of Antibiotics in Water, Suspended Solid, Bottom Mud, in Semi-enclosed Sea and Its Accumulation in Plankton in Semi-enclosed Se

Yukihiro Munekage、Nozomu Iwasaki、Tuan Xu Le、Hiroshi Hasekawa、Yuki Natukawa、Sakiko Kikuhara：“半封闭海水中抗生素在水中、悬浮固体、底泥中的分布及其在半封闭海水中浮游生物中的积累的研究

Tuan Xuan Le, Yukihiro Munekage, Dao Anh Thi Phan, Dao Quynh, Thi Quan: "The Environmental Quality of Shrimp Ponds in Mangrove Areas"Proceedings of 13^<th>(2003) International Offshore and polar Engineering Conference IS0PE-2003. 13. (2003)

Tuan Xu Le，Yukihiro Munekage，Dao Anh Thi Phan，Dao Quynh，Thi Quan：“红树林地区虾塘的环境质量”第 13 届（2003 年）国际近海和极地工程会议 IS0PE-2003 论文集。

宗景志浩, LE Xuan Tuan, 夏川優樹, 岩崎望: "内湾域における残留化学物質の分布と生物濃縮に関する研究"海岸工学論文集. 49. 1096-1100 (2002)

Shihiro Munekage，LE Xu Tuan，Yuki Natsukawa，Nozomi Iwasaki：“内湾地区残留化学物质的分布和生物浓缩研究”海岸工程杂志49。1096-1100（2002）。

Phan Thi Anh Dao, Le Xuan Tuan, Takara Kaoru, Munekage Yukihiro: "Water Resources Management in Estuarine Areas"First International Conference on Hydrogy and Water resources in Asia Pacific Region APHW2003. 1-2. 977-983 (2003)

Phan Thi Anh Dao、Le Xu Tuan、Takara Kaoru、Munekage Yukihiro：“河口地区水资源管理”亚太地区第一届水文学和水资源国际会议 APHW2003。