Analysis of receptor carbohydrates for Helicobacter pylori from human fluds

为了研究人类幽门螺杆菌的受体分子，本研究包括：1)比较了幽门螺杆菌与固定化鞘糖脂（GSLs）的直接粘附及其相应的寡糖共轭新糖缀合物。这些细菌被发现能附着在硫脂、GM3、GalCer和LacCer上，但不能附着在神经酰胺、鞘磷脂或与β-半乳糖、乳糖、3'-唾液乳糖或3'-磺基β-半乳糖偶联的聚丙烯酰胺上。此外，具有相应低聚糖的新糖脂或bsa衍生物不能作为配体。与α-羟基脂肪酸的GalCer相比，幽门螺杆菌对α-羟基脂肪酸的粘附力要强得多。2)当幽门螺杆菌直接粘附于固定化人体体液时，临床分离的菌株均对固定化人体胃液有较强的粘附。另一方面，5株菌株中有2株与leb抗原不粘附，1株与硫脂不粘附。将固定在平板上的胃液经高酸盐氧化后，幽门螺杆菌对人胃液的粘附力显著降低，而唾液酸酶处理后，幽门螺杆菌对胃液的粘附力不受影响，说明胃液中的受体是非唾液酸化的糖缀合物。此外，低聚糖、硫脂和硫酸化低聚糖对胃液的粘附没有抑制作用。

In order to investigate the receptor molecules for H.pylori in human, this study involved1)Direct adhesion of Helicobacter pylori to immobilized glycosphingolipids (GSLs) was compared to that of their corresponding oligosaecharide-conjugated neoglycoconjugates. "These bacteria were found to adhere to sulfatide, GM3, GalCer and LacCer, but not to" "ceramide, sphingomyelin, or polyacrylamides conjugated with β-galactose, lactose, 3'-" "sialyllactose or 3'-sulfo-β-galactose. Furthermore, neoglycolipids or BSA-derivatives" with corresponding oligosaccharides were unable to serve as the ligands. H. pylori adhesion to GalCer with α-hydroxyl fatty acid was much stronger than GalCer with the non-hydroxyl fatty acid.2) When direct adhesion of H. pylori to immobilized human fluids, all of the clinical "isolated strains strongly adhered to immobilized human gastric juice. On the other hand, " 2 of 5 strains did not adhered to Leb-antigen and one strain did not adhered to sulfatide. The adhesion of H. pylori to human gastric juice remarkably reduced when gastric juice "immobilized on the plate were oxidized by periodate, but that was not affected by the" "treatment with sialidase, indicating that the receptors in gastric juice are non-sialylated" "glycoconjugates. In addition, Leb-oligosaccharides, sulfatide, and sulfated" oligosaccharides did not inhibit the adhesion to gastric juice.