Analysis of receptor carbohydrates for Helicobacter pylori from human fluds

为了研究幽门螺杆菌在人体内的受体分子，本研究1)比较了幽门螺杆菌与固定化糖鞘糖脂(GSLS)的直接黏附及其相应的低聚糖连接的新糖偶联物的黏附。这些细菌能附着于硫脂、GM3、GalCer和LacCer，但不能附着于神经酰胺、鞘磷脂或与β-半乳糖、乳糖、3‘-唾液酸乳糖或3’-磺酸-β-半乳糖结合的聚丙烯酰胺。此外，含有相应寡糖的新糖脂或BSA衍生物不能作为配体。用α-羟基脂肪酸与GalCer的粘附性明显强于与非羟基脂肪酸的粘附性。2)Hp与固定化人胃液直接粘附时，临床分离株均对固定化人胃液有较强的粘附性，而5株菌株中有2株不与Leb抗原结合，1株不与硫脂结合。用高碘酸氧化固定在平板上的胃液后，幽门螺杆菌对人胃液的粘附率显著降低，但不受唾液酸酶处理的影响，说明胃液中的受体是非唾液酸化的糖结合物，此外，Leb-寡糖、硫代低聚糖和硫酸低聚糖不能抑制幽门螺杆菌与人胃液的粘附性。

In order to investigate the receptor molecules for H.pylori in human, this study involved1)Direct adhesion of Helicobacter pylori to immobilized glycosphingolipids (GSLs) was compared to that of their corresponding oligosaecharide-conjugated neoglycoconjugates. "These bacteria were found to adhere to sulfatide, GM3, GalCer and LacCer, but not to" "ceramide, sphingomyelin, or polyacrylamides conjugated with β-galactose, lactose, 3'-" "sialyllactose or 3'-sulfo-β-galactose. Furthermore, neoglycolipids or BSA-derivatives" with corresponding oligosaccharides were unable to serve as the ligands. H. pylori adhesion to GalCer with α-hydroxyl fatty acid was much stronger than GalCer with the non-hydroxyl fatty acid.2) When direct adhesion of H. pylori to immobilized human fluids, all of the clinical "isolated strains strongly adhered to immobilized human gastric juice. On the other hand, " 2 of 5 strains did not adhered to Leb-antigen and one strain did not adhered to sulfatide. The adhesion of H. pylori to human gastric juice remarkably reduced when gastric juice "immobilized on the plate were oxidized by periodate, but that was not affected by the" "treatment with sialidase, indicating that the receptors in gastric juice are non-sialylated" "glycoconjugates. In addition, Leb-oligosaccharides, sulfatide, and sulfated" oligosaccharides did not inhibit the adhesion to gastric juice.