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Analysis of receptor carbohydrates for Helicobacter pylori from human fluds

人类体液中幽门螺杆菌受体碳水化合物的分析

基本信息

批准号：
12680619
负责人：
KOJIMA Naoya
金额：
$ 2.37万
依托单位：
TOKAI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

项目摘要

In order to investigate the receptor molecules for H.pylori in human, this study involved1)Direct adhesion of Helicobacter pylori to immobilized glycosphingolipids (GSLs) was compared to that of their corresponding oligosaecharide-conjugated neoglycoconjugates. "These bacteria were found to adhere to sulfatide, GM3, GalCer and LacCer, but not to" "ceramide, sphingomyelin, or polyacrylamides conjugated with β-galactose, lactose, 3'-" "sialyllactose or 3'-sulfo-β-galactose. Furthermore, neoglycolipids or BSA-derivatives" with corresponding oligosaccharides were unable to serve as the ligands. H. pylori adhesion to GalCer with α-hydroxyl fatty acid was much stronger than GalCer with the non-hydroxyl fatty acid.2) When direct adhesion of H. pylori to immobilized human fluids, all of the clinical "isolated strains strongly adhered to immobilized human gastric juice. On the other hand, " 2 of 5 strains did not adhered to Leb-antigen and one strain did not adhered to sulfatide. The adhesion of H. pylori to human gastric juice remarkably reduced when gastric juice "immobilized on the plate were oxidized by periodate, but that was not affected by the" "treatment with sialidase, indicating that the receptors in gastric juice are non-sialylated" "glycoconjugates. In addition, Leb-oligosaccharides, sulfatide, and sulfated" oligosaccharides did not inhibit the adhesion to gastric juice.

为了研究幽门螺杆菌在人体内的受体分子，本研究包括：1）比较幽门螺杆菌与固定化鞘糖脂（glycosphingolipids，GSL）及其相应的寡糖缀合物（oligosaecharide conjugated neoglycoconjugates）的直接粘附。发现这些细菌粘附于硫苷脂、GM 3、GalCer和LacCer，但不粘附于神经酰胺、鞘磷脂或与β-半乳糖、乳糖、3 '-”“唾液酸乳糖或3'-磺基-β-半乳糖结合的聚丙烯酰胺。此外，具有相应寡糖的新糖脂或BSA衍生物不能作为配体。H. pylori与含α-羟基脂肪酸的GalCer的粘附作用明显强于含非羟基脂肪酸的GalCer。pylori与固定化人胃液的粘附试验结果表明，所有临床分离菌株均与固定化人胃液有很强的粘附。另一方面，“5个菌株中有2个不粘附Leb抗原，1个菌株不粘附硫苷脂。H. pylori对人胃液的受体在高碘酸氧化后明显降低，但不受唾液酸酶处理的影响，表明胃液中的受体是非唾液酸化的糖缀合物。此外，Leb-寡糖、硫苷脂和硫酸化寡糖不抑制对胃液的粘附。