Characterization of a novel function of gicerin, a cell adhesion nolecule, in the mature nervous system

细胞粘附分子甘油在成熟神经系统中的新功能的表征

• 批准号: 12680753
• 负责人: TAIRA Eiichi
• 金额: $ 2.62万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680753/
• 关键词: Cell adhesion molecule; Genomic cloning; Regulation of gene expression; Neurite promotion; 免疫グロブリンスーパーファミリー; 細胞内領域; 神経突起; 微絨毛; 細胞接着; 海馬; 脊髄後根神経節; 細胞骨格

1) Characterization of gicerin geneWe have isolated 16kbp of mouse gicerin gene and determined the sequence. We clarified that the gicerin gene is organized by 16 exons and two isoforms are generated by an alternative splicing of exon 15. Next we determined the promoter region of gicerin gene and determined a responsible domain for growth factor stimulation. The domain was also confirmed by a gel reterdation assay.2) Analysis of gicerin function in the neurite promotion from PC12 cells.We clarified that increase of gicerin expression in PC 12 cells enhanced neurite promotion.3) Generation of transgenic mouse, which constitutively express gicerin in nerve cellsWe established three lines of transgenic mouse, which express gicerin in nerve cells. In order to establish them, we introduced gicerin cDNA with neurofilament promoter as well as GFP with neurofilament promoter.

1) gicerin基因的鉴定我们分离了小鼠gicerin基因16kbp，并确定了序列。我们澄清了gicerin基因由16个外显子组成，两个异构体是由外显子15的选择性剪接产生的。接下来，我们确定了gicerin基因的启动子区域，并确定了一个负责生长因子刺激的区域。该结构域也通过凝胶还原实验得到了证实。2)分析gicerin在PC12细胞神经突促进中的作用。我们阐明了pc12细胞中gicerin表达的增加促进了神经突的促进。3)在神经细胞中组成表达gicerin的转基因小鼠的产生我们建立了3个在神经细胞中表达gicerin的转基因小鼠系。为了建立它们，我们引入了带神经丝启动子的gicerin cDNA和带神经丝启动子的GFP。

项目成果

E.Taira, Y.Tsukamoto, K.Kohama, M.Maeda, H.Kiyama, N.Miki: "Expression and involvement of gicerin, a cell adhesion molecule, in the development of chick optic tectum."J.Neurochem.. 88. 891-899 (2004)

E.Taira、Y.Tsukamoto、K.Kohama、M.Maeda、H.Kiyama、N.Miki：“细胞粘附分子 gicerin 在鸡视顶盖发育中的表达和参与。”J.Neurochem..

S.Okumura, K.Kohama, S.Kim, H.Iwao, N.Miki, E.Taira: "Induction of gicerin/CD146 in the rat carotid artery after balloon injury."Biochem.Biophys.Res.Comm.. 3/3. 902-906 (2004)

S.Okumura、K.Kohama、S.Kim、H.Iwao、N.Miki、E.Taira：“球囊损伤后大鼠颈动脉中甘油/CD146的诱导。”Biochem.Biophys.Res.Comm.. 3

E.Taira, K.Kohama, Y.Tsukamoto, S.Okumura, N.Miki: "Characterization of gicerin/MUC18/CD146in the rat nervous system."J.Cell.Physiol.. 198. 377-387 (2004)

E.Taira、K.Kohama、Y.Tsukamoto、S.Okumura、N.Miki：“大鼠神经系统中甘油/MUC18/CD146 的表征。”J.Cell.Physiol.. 198. 377-387 (2004)

E.Kumamaru, C-H.Kuo, T.Fujimoto, K.Kohama, L-H.Zeng, E.Taira et al.: "Reticulon3 expression in rat optic and lofactory systems."Neurosci.Lett.. 356. 17-20 (2004)

E.Kumamaru、C-H.Kuo、T.Fujimoto、K.Kohama、L-H.Zeng、E.Taira 等：“大鼠视神经和嗅觉系统中的 Reticulon3 表达。”Neurosci.Lett.. 356. 17-20 (2004

S.Tsuchiya, Y.Tsukamoto, M.Furuya, S.Hiroi, N.Miki, F.Sasaki, E.Taira: "Gicerin promotes the metastasis of MDCC-MSB1 cells derived from Marek disease lymphoma."Cell Tissue Res.. (in press). (2004)

S.Tsuchiya、Y.Tsukamoto、M.Furuya、S.Hiroi、N.Miki、F.Sasaki、E.Taira：“Gicerin 促进源自马立克病淋巴瘤的 MDCC-MSB1 细胞的转移。”细胞组织研究。