Construction of congenic strains from epileptic model rat NER

癫痫模型大鼠NER同源品系的构建

• 批准号: 12680810
• 负责人: KITADA Kazuhiro
• 金额: $ 2.3万
• 依托单位: Hokkaido University (2002-2003)Kyoto University (2000-2001)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680810/
• 关键词: epilepsy; animal model; NER; genetic analysis; congenic strain; 疾患モデル動物; コンジェニック系統; 対照系統

NER is an animal model of epilepsy that exhibit spontaneous tonic-clonic convulsions, beginning with neck and forelimb clonus, wild jumping/running, opisthotonic posturing, and evolving to tonic, then clonic convulsion, followed by postictal flaccidity. Ictal cortical and hippocampal EEGs are characterized by high-voltage spikes followed by diffuse spike-and-wave or polyspike-and-wave complexes. Seizure susceptibility to pentylenetetrazol, tossing, and transcorneal electroshock, but not to tactile, photic, or acoustic stimuli, or to transauricular electroshock. No pathologic changes were demonstrated in the CNS. Spontaneous convulsions are controlled by a major autosomal recessive gene for epilepsy, that are comparable to generalized tonic-clonic seizures in humansIn this project, we have generated two congenic lines of F344.NER-ner1^<NER>/ner1^<NER> and NER.F344-ner1^<F344>/ner1^<F344>. The former is expected to to show GTC, and the latter is expected not to show GTC. In the future, it is considered that strict animal experiment for reseach on epilepsy will be possible using NER, F344 and these 2 lines of congenic strains.

NER是一种癫痫动物模型，表现为自发的强直-阵挛痉挛，始于颈部和前肢阵挛，疯狂跳跃/奔跑，反张姿势，演变为强直，然后阵挛痉挛，然后是死后松弛。发作期皮质和海马区脑电以高电压棘波和弥漫性棘波或多棘波复合波为特征。癫痫对戊四氮、翻腾和经角膜电击的敏感性，但对触觉、光或声刺激或经耳电刺激不敏感。中枢神经系统未见病理改变。自发性惊厥是由癫痫的一个主要常染色体隐性基因控制的，这与人类全身性强直-克隆性发作类似。在这个项目中，我们产生了两个同源基因系F344.NER-ner1^&lt；Ner&gt；/ner1^&lt；Ner&gt；和NER.F344&gt；/ner1^&lt；F344&gt；前者预计会显示GTC，后者预计不会显示GTC。在未来，利用NER、F344和这2株同源菌株进行癫痫的动物实验研究是可能的。

项目成果

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