Relationship between mucosal immune response in suncus tonsil and onset of focal infection

太阳扁桃体粘膜免疫反应与局灶性感染发生的关系

• 批准号: 12680818
• 负责人: TOHYA Kazuo
• 金额: $ 2.05万
• 依托单位: Kansai College of Oriental Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680818/
• 关键词: suncus; mucosal immunity; platine tonsil; glomerulus; IgA; specific antibody formation; 耳管扁桃

This study was performed to investigate the relationship between mucosal immune response in suncus palatine tonsil and the onset of tonsillar focal infection at the immunohistological and immunopathological points of view, in 2000 to 2001.The tonsils showed typical structures as mucosa-associated iymphoid tissues at the level of light and electron microscopy. Trial for isolation and purification of IgA-fraction from the extract of gastrointestinal tracts of suncus was incomplete, however, the immunohistochemical staining of the tonsils clearly showed that the tissues revealed a higher activity with regard to IgA production at the site of the tonsillar crypt epithelium, rather than IgG or IgM production. Immunization of antigen with mucosal immune adjuvant to the tonsils induced specific antibody formation within the immuned tonsils and their regional lymph nodes. Interestingly, some tonsillar responder animals had antibody-specific deposits in their glomerurus with segmental or membranous patterns, but they did not show any sign of mesangial proliferation or crescerlt formation. Although there is no direct evidence between tonsillar IgA production and the glomerulonephritis, the obtained results suggest that the deposition of the immune complex in pathological glomerulus may be induced by the circulating IgA derived from the antigen-stimulated tonsils, showing the organs play an important role as not only inductive site but also effector site of local immune response. Moreover, it is suggested that the suncus is useful experimental animal for research of the onset mechanism of tonsillar focal infection.

本研究从免疫组织学和免疫病理学的角度探讨了2000 ~ 2001年腭子扁桃体粘膜免疫反应与扁桃体局灶性感染发生的关系。扁桃体在光镜和电镜下表现为典型的粘膜相关淋巴组织结构。从菟丝子胃肠道提取物中分离和纯化IgA组分的试验尚不完整，然而，扁桃体的免疫组织化学染色清楚地表明，扁桃体隐窝上皮部位的IgA产生活性较高，而不是IgG或IgM的产生。抗原与粘膜免疫佐剂免疫扁桃体诱导特异性抗体在免疫扁桃体及其区域淋巴结内形成。有趣的是，一些扁桃体应答动物的肾小球有抗体特异性沉积，呈节段性或膜性，但它们没有显示任何系膜增殖或新月形成的迹象。虽然扁桃体IgA的产生与肾小球肾炎之间没有直接的证据，但上述结果提示病理性肾小球中免疫复合物的沉积可能是由抗原刺激的扁桃体产生的循环IgA诱导的，表明该器官不仅是局部免疫反应的诱导部位，也是局部免疫反应的效应部位。同时，为研究扁桃体局灶性感染的发病机制提供了实验动物依据。