Evolution of photosynthetic oxygen-evolving system from the structure and function of extrinsic proteins

从外源蛋白的结构和功能看光合放氧系统的进化

• 批准号: 13640658
• 负责人: ENAMI Isao
• 金额: $ 1.86万
• 依托单位: Tokyo University of Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13640658/
• 关键词: Extrinsic protein; 33 kDa protein; Evolution; Oxygen evolution; Cleavage sites; Chemical modification; Positive charge; Structure; 33kDa蛋白; 酸素発生系; プロテアーゼ切断部位; リジン; 光合成; プロテアーゼ処理; 蛋白の構造

(1) The psbO gene encoding the extrinsic 33 kDa protein of oxygen-evolving photosystem II (PSII) complex was cloned and sequenced from a red alga, Cyanidium caldarium. (2) The cleavage sites of the 33kDa protein by chymotrypsin or V8 protease were determined and compared among a cyanobacterium (Synechococcus elongatus), a euglena (Euglena gracilis), a green alga (Chlamydomonas reinhardtii) and two higher plants (Spinacia oleracea and Oryza sativa). The cleavage sites by chymotrypsin were at 156F and 190F for the cyanobacterium, 159M, 160F and 192L for red alga, 11Y and 151F for euglena, 10Y and 150F for green alga, and 16Y for apinach, respectively. The cleavage sites by V8 protease were at 181E(cyanobacterium), 182E and 195E (red alga), 13E, 67E, 69E, 153D and 181E (euglena), 176E and 180E (green alga), and 18E or 19E (higher plants). In terms of the cleavage sites, the structure of the 33 kDa protein can be divided into three major groups: Cyanobacterial and red algal-type has cleavage sites at residues around 156-195, higher plant-type at residues 16-19, and euglena and green algal-type at residues of both cyanobacterial and higher plant-types

(1)从一种红藻Cyanidium caldarium中克隆了编码光合放氧系统II（PSII）复合物外源蛋白的psbO基因，并进行了序列测定。(2)测定并比较了细长聚球藻（Synechococcus elongatus）、纤细裸藻（Euglena gracilis）、莱茵衣藻（Chlamydellae reinhardtii）和两种高等植物（菠菜和水稻）对33kDa蛋白的糜蛋白酶和V8蛋白酶切割位点。胰凝乳蛋白酶的切割位点分别为：蓝藻156 F和190 F，红球藻159 M、160 F和192 L，裸藻11 Y和151 F，绿色藻10 Y和150 F，以及Apinach 16 Y。V8蛋白酶的切割位点分别位于181 E（蓝藻）、182 E和195 E（红球藻）、13 E、67 E、69 E、153 D和181 E（裸藻）、176 E和180 E（绿色藻）以及18 E或19 E（高等植物）。根据切割位点，33 kDa蛋白的结构可以分为三大类：蓝藻和红藻型在156 - 195残基附近具有切割位点，高等植物型在16 - 19残基处具有切割位点，裸藻和绿色藻型在蓝藻和高等植物型的残基处都具有切割位点

项目成果

Takehiro Suzuki, Jun Minagawa, Tatsuya Tomo, Kintake Sonoike, Hisataka Ohta and Isao Enami: "Binding and functional properties of the extrinsic proteins in oxygen-evolving Photosystem II particle from a green alga, Chlamydomonas reinhardtii having His-tag

Takehiro Suzuki、Jun Minakawa、Tatsuya Tomo、Kintake Sonoike、Hisataka Ohta 和 Isao Enami：“来自绿藻、莱茵衣藻（具有 His 标签）的释氧光系统 II 颗粒中外在蛋白的结合和功能特性

Hisataka Ohta, Akinon Okumura, Satoshi Okuyama, Ai Akiyama, Masako Iwai, Shizue Yoshihara, Jian-Ren Shen, Masaharu and Isao Enami: "Cloning Expression of the psbU Gene and Functional Studies of Recombinant 12kDa Protein of Photosystem II from a Red Alga C

Hisataka Ohta、Akinon Okumura、Satoshi Okuyama、Ai Akiyama、Masako Iwai、Shizue Yoshihara、Jian-Ren Shen、Masaharu 和 Isao Enami：“红藻 C 光系统 II 重组 12kDa 蛋白的 psbU 基因的克隆表达和功能研究

Sadahiro Kamiya, Rina Kato, Masayoshi Wakabayashi, Takehiro Tohyama, Isao Enami, Masaaki Ueki, Hirofumi Yajima, Tadahiro Ishii, Hiroshi Nakamura, Takashi Katayama Junichi Takagi, Fumio Fukai: "Fibronectin peptides derived from two distinct regions stimula

Sadahiro Kamiya、Rina Kato、Masayoshi Wakabayashi、Takehiro Tohyama、Isao Enami、Masaaki Ueki、Hirofumi Yajima、Tadahiro Ishii、Hiroshi Nakamura、Takashi Katayama Junichi Takagi、Fumio Fukai：“源自两个不同区域刺激的纤连蛋白肽

A.Tohri, I.Enami et al: "Comparison of the structure of the extrinsic 33 kDa protein from different organisms"Plant and Cell Physiology. (in press). (2002)

A.Tohri、I.Enami 等人：“来自不同生物体的外源 33 kDa 蛋白质的结构比较”植物和细胞生理学。

Isao Enami, Shizue Yashihara, Hisataka Ohta and Jian-Ren Shen: "Cross-reconstitution of four extrinsic from a red alga higher plant and cyanobacterial PSII."Photosynthesis. Mechanisms and Effects. Vol.II. 1435-1438 (1999)

Isao Enami、Shizue Yashihara、Hisataka Ohta 和 Ken-Ren Shen：“红藻高等植物和蓝藻 PSII 的四种外源物的交叉重建。”光合作用。