Purification of gonad-stimulating substance (GSS) and its secretion mechanism during breeding season
繁殖期性腺刺激物质(GSS)的纯化及其分泌机制
基本信息
- 批准号:13640673
- 负责人:
- 金额:$ 1.79万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In starfish, oocyte maturation and spawning are first triggered by a peptide hormone (gonad-stimulating substance, GSS) present in nervous tissue. GSS was purified from radial nerves of starfish Asterina pectinifera. Although a molecular weight of A. pectinifera GSS was estimated to about 4,700 using a mass-spectrometry, the quantity was not enough to determine the amino acid sequence. This study also examined organization of neural networks in ovaries and testes of the starfish, Asterina pectinifera by employing an antibody that recognizes molecules belonging to SALMFamide family of echinoderm neuropeptides. Following incubation with a SALMFamide 1 antibody primary antibody, and subsequently with rhodamine-conjugated second antibody identified the network structures were observed in both ovaries and testes. The networks were restricted mainly to their surface and little evidence of immunoreactivity was seen inside their basement membranes. The result suggests that neural networks distributed in gonads maybe involved in the regulation of gametogenesis and gamete shedding in starfish.Furthermore, using degenerate primers derived from consensus sequences of the memmalian glycoprotein hormone (LH, FSH and TSH) receptors, we cloned cDNAs (apGPHR) from ovaries of.the starfish Asterina pectinifera. However, a partially purified gonad-stimulating substance (GSS) failed to increase cyclic AMP levels in COS-7 cells expressing apGPHR. This suggests that a new neural petide other than GSS is a ligand for the apGPHR in starfish.
在海星中,卵母细胞成熟和产卵首先由神经组织中存在的肽激素(性腺刺激物质,GSS)触发。GSS是从海星(Asterina pectinifera)的桡神经中分离纯化的。虽然A.使用质谱法估计pectinifera GSS为约4,700,该量不足以确定氨基酸序列。本研究还研究了海星,海星的卵巢和睾丸中的神经网络的组织,采用抗体识别分子属于SALMFamide家庭棘皮动物神经肽。与SALMF酰胺1抗体一抗孵育后,随后与罗丹明缀合的二抗孵育,在卵巢和睾丸中观察到网络结构。这些网络主要局限于其表面,在其基底膜内几乎看不到免疫反应性的证据。本研究还利用膜糖蛋白激素(LH、FSH和TSH)受体的保守序列设计简并引物,从栉孔海星(Asterina pectinifera)卵巢中克隆了apGPHR基因。然而,部分纯化的性腺刺激物质(GSS)未能增加表达apGPHR的COS-7细胞中的环AMP水平。这表明,一个新的神经肽以外的GSS是海星的apGPHR的配体。
项目成果
期刊论文数量(15)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Masatoshi Mita: "Monduzzi Editore, Bologna, Italy"Proceedings of the 21th Conference of European Comparative Endocrinologists. 543 (2002)
Masatoshi Mita:“Monduzzi Editore,博洛尼亚,意大利”第 21 届欧洲比较内分泌学家会议论文集。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masatoshi Mita: "Role of ecto-ATP diphosphohydrolase in ovarian follocle cells of the starfish Asterina pectinifera"Zoological Science. 18. 551-557 (2001)
Masatoshi Mita:“外源 ATP 二磷酸水解酶在海星海星卵巢滤泡细胞中的作用”动物学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masatoshi Mita: "Role of ecto-ATP diphosphohydrolase in ovarian follicle cells of the starfish Asterina pectinifera"Zool.Sci.. 18. 551-557 (2001)
Masatoshi Mita:“外源ATP二磷酸水解酶在海星海星卵巢卵泡细胞中的作用”Zool.Sci.. 18. 551-557 (2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masatoshi Mita: "Interaction of N1-substituted adenines with 1-methyladenine receptors of starfish oocytes in induction of maturation"Comparative Biochemistry and Physiology Part B. 130. 427-434 (2001)
Masatoshi Mita:“N1-取代腺嘌呤与海星卵母细胞1-甲基腺嘌呤受体在成熟诱导中的相互作用”比较生物化学和生理学部分B.130.427-434(2001)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masatoshi Mita: "cDNA cloning of a novel member of the glycoprotein hormone receptor family from a starfish Asterina pectinifera"Molecular Reproduction and Development. (印刷中). (2003)
Masatoshi Mita:“海星糖蛋白激素受体家族新成员的 cDNA 克隆”《分子复制与发育》(出版中)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MITA Masatoshi其他文献
MITA Masatoshi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MITA Masatoshi', 18)}}的其他基金
Action of starfish gonadotropic hormone: interaction with the receptor and mechanism of gamete shedding
海星促性腺激素的作用:与受体的相互作用和配子脱落的机制
- 批准号:
19K06747 - 财政年份:2019
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study of mechanism in starfish oocyte maturation induced by a novel identified gonad-stimulating hormone
新型性腺刺激激素诱导海星卵母细胞成熟机制的研究
- 批准号:
21570063 - 财政年份:2009
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study of action mechanism of gonad-stimulating hormone (GSS) in starfish
海星促性腺激素(GSS)作用机制的研究
- 批准号:
18570056 - 财政年份:2006
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Study of gonad-stimulating substance (GSS) and 1-methyladenine which are related to starfish oocyte maturation
与海星卵母细胞成熟相关的性腺刺激物质(GSS)和1-甲基腺嘌呤的研究
- 批准号:
11640672 - 财政年份:1999
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Biosynthesis of maturation-inducing hormone (1-methyladenine) by starfish ovarian follicle cells and its relation to cell adhesion factor
海星卵泡细胞成熟诱导激素(1-甲基腺嘌呤)的生物合成及其与细胞粘附因子的关系
- 批准号:
09640797 - 财政年份:1997
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似海外基金
Study of gonad-stimulating substance (GSS) and 1-methyladenine which are related to starfish oocyte maturation
与海星卵母细胞成熟相关的性腺刺激物质(GSS)和1-甲基腺嘌呤的研究
- 批准号:
11640672 - 财政年份:1999
- 资助金额:
$ 1.79万 - 项目类别:
Grant-in-Aid for Scientific Research (C)