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Mechamisms of gene expression and physiological function of bombyxin

家蚕素基因表达机制及生理功能

基本信息

批准号：
13660058
负责人：
IWAMI Masafumi
金额：
$ 2.18万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660058/
关键词：
bombyxin insulin transcription element Bombyx mori brain neurosecretory cell multi-gene family gene transfer

项目摘要

1. Bombyxin genes of the silkmoth Bombyx mori encode insulin-related peptides and are expressed in four pairs of the neurosecretory cells in pars intercerebralis of brain. We identified two transcription elements for the cell-specific expression of bombyxin F1 gene. One of the elements, BOSE, was essential for the cell-specific expression and localized in the region from -170 to -159 bp upstream of the translation start site of the F1 gene. The other element, activator element, was responsible for increasing the expression level and located in the region from -185 to -181 bp.2. The expression of bombyxin genes showed a gene-arrangement-dependent manner. The family A and C genes expressed are located exclusively in the gene pairs but not in the gene triplets. The family B genes belonging to the gene triplets are expressed. In the gene pairs, some family B genes are expressed, whereas the other family B genes are not expressed. By reporter gene analysis using various bombyxin gene promoters, we confirmed the gene-arrangement-dependent manner of the expression of the bombyxin family A and C genes. The expression manner of bombyxin genes are unique but the mechanisms of the expression remains to be elucidated.3. We examined the silkworm baculovirus expression vector system for the in vivo expression of the GFP gene under the control of several gene promoters, including the bombyxin promoter and the prothoracicotropic hormone (PTTH) gene promoter. Depending on the neurohormone gene promoters used, the GFP gene is correctly and specifically expressed in the neurosecretory cells of the brain. This result indicates that thesilkworm baculovirus expression vector system can serve as an efficient system permitting gene delivery into neural tissues in insects.

1.家蚕胰岛素相关肽基因编码的胰岛素相关肽在大脑中脑部的4对神经分泌细胞中表达。我们确定了两个转录元件的细胞特异性表达的家蚕毒素F1基因。其中一个元件BOSE是细胞特异性表达所必需的，位于F1基因翻译起始位点上游-170 ~-159bp的区域。另一个元件是激活元件，位于-185 ~-181bp之间，是表达量增加的主要元件。家蚕毒素基因的表达具有基因排列依赖性。表达的家族A和C基因仅位于基因对中，而不位于基因三联体中。表达属于基因三联体的家族B基因。在基因对中，一些家族B基因表达，而其他家族B基因不表达。通过报告基因分析，使用不同的家蚕毒素基因启动子，我们证实了基因排列依赖的方式表达的家蚕毒素家族A和C基因。家蚕毒素基因表达方式独特，但其表达机制尚待阐明.我们研究了家蚕杆状病毒表达载体系统的GFP基因在几个基因启动子，包括家蚕毒素启动子和促前胸腺激素（PTTH）基因启动子的控制下，在体内表达。根据所使用的神经激素基因启动子，GFP基因在脑的神经分泌细胞中正确且特异地表达。这一结果表明，家蚕杆状病毒表达载体系统可以作为一个有效的系统，允许基因传递到昆虫的神经组织。