Biosynthesis of phytotoxins in phytopathogenic fungi

植物病原真菌中植物毒素的生物合成

• 批准号: 13660108
• 负责人: NAKAJIMA Hiromitsu
• 金额: $ 2.24万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660108/
• 关键词: Phytopathogens; Neocosmospora vasinfecta; reduction enzyme; phytotoxins; SDS-PAGE; biosynthesis; native PAGE; purification; ポリケタイド; 生合成酵素; SDSPAGE; ポリケチド; 酵素タンパク; 分子量; Km値

Neovasifuranones are phytotoxins produced by the phytopathogenic fungus Neocosmospora vasinfecta NHL2298. Recently conversion of neovasifuranone A aldehyde into neovasifuranone A was observed in the cell-free system obtained from this fungus. We studied on the properties of this of this conversion enzyme(s)and found that there are two enzymes involved in this conversion step. We determined the molecular weight, optimal pH, optimal temperature, Km and Vmax values of these two enzymes. We also purified one of these enzymes by centrifugation, DE-52,Sephacryl S-300, TSKgel G3000SW and Mono Q column chromatographies. The purest sample showed one major protein band around 40kDa on SDS-PAGE analysis. This band protein was determined to be an enzyme protein after native PAGE and enzyme assay.

新氟烷酮是由植物病原真菌Neocosmospora vasinfeta NHL2298产生的植物毒素。最近在该真菌的无细胞体系中观察到新氟西酮A醛转化为新氟西酮A。我们研究了这种转化酶的性质，发现有两种酶参与了这一转化步骤。测定了这两种酶的分子量、最适pH、最适温度、Km和Vmax值。我们还通过离心、DE-52、Sephacryl S-300、TSKgel G3000SW和Mono Q柱层析纯化了其中一种酶。在SDS-PAGE分析中，纯度最高的样品显示一个约40kDa的主要蛋白带。经天然PAGE和酶分析，该条带蛋白为酶蛋白。

项目成果

K.Yabe et al.: "Enzymatic conversion of averufin to hydroxyversicolorone and elucidation of a novel metabolic grid involved in alfatoxin biosynthesis"Appl. Environ. Microbiol.. 69巻. 66-73 (2003)

K. Yabe 等人：“阿维芦芬到羟基杂色酮的酶促转化以及黄曲霉毒素生物合成中涉及的新型代谢网格的阐明”Appl Environ Microbiol.. 69. 66-73 (2003)

H.Tani, Y.Fujii, H.Nakajima: "Chlamydocin analogues from the soil fungus Peniophora sp.: structures and plant growth-retardant activity"Phytochemistry. 58(2). 305-310 (2001)

H.Tani、Y.Fujii、H.Nakajima：“来自土壤真菌 Peniophora sp. 的衣霉素类似物：结构和植物生长抑制活性”植物化学。

Y.Fujii et al.: "Fungal melanin inhibitor and related compounds from Penicillium decumbens"Phytochemistry. 60巻. 703-708 (2002)

Y.Fujii 等人：“来自斜卧青霉的真菌黑色素抑制剂和相关化合物”植物化学 60. 703-708 (2002)。

H.A.A Babiker, H.Nakajima, S.Inanaga, Y.Sugimoto: "Series Recent Research Progress in Medicinal Plants, Vol.4-Biotechnology and Genetic Engineering (eds.J.N.Govil, P.Anand Kumar and V.K, Singh)"SCI TECH Pub., USA. 163-174 (2002)

H.A.A Babiker、H.Nakajima、S.Inanaga、Y.Sugimoto：“药用植物系列最新研究进展，第 4 卷-生物技术和基因工程（编辑 J.N.Govil、P.Anand Kumar 和 V.K、Singh）”SCI TECH

H.Tani et al.: "A plant growth retardant related to chlamydocin and its proposes mechanism of action"Phytochemistry. 62巻. 1133-1140 (2003)

H. Tani 等人：“与衣霉素相关的植物生长抑制剂及其提出的作用机制”《植物化学》62. 1133-1140 (2003)。