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Analyses of growth performance by fluorescent substances and trace elements in mollusks

荧光物质和微量元素对软体动物生长性能的分析

基本信息

批准号：
13660189
负责人：
OKOSHI Kenji
金额：
$ 2.3万
依托单位：
Ishinomaki Senshu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660189/
关键词：
shell age determination growth analysis trace element fluorescent substance strontium chloride tetracycline bivalve

项目摘要

Biomineralization research is one of the more important research subjects from both a fundamental and applied point of view. The molluscan shells contain considerable information about their own growth history including the continuous condition of mineralization and stress or disease. Analysis of this information is a factor of great importance both in marine biology and in aquaculture. The application of fluorescent substances to the analysis of growth performance and hard tissue formation is popular in medical and dental sciences. The fish otoliths also can be marked by immersion in some fluorescent substances. The object of this study is to develop a method of marking the hard tissues of mollusks such as shell and pearl of bivalves with fluorescent substances in order to obtain effective information on hard tissue formation. To determine the optimum tetracycline (TC) and alizarine complexone (ALC) concentration and treatment time for marking the shells of oyster Crassostrea gigas various combinations of concentration and treatment time were tested. TC presented a clear yellow fluorescent line in the dorso-ventral section of oyster shells under UV light. The optimum condition for treatment was 200mg/l for 12-24 h. Orange mark was observed by ALC treatment at 150-300mg/l for 12-24 h. Double treatments by TC and by ALC were successful. Similarity and difference of growth performance between the shell and the pearl can be observed in the pearl oyster Pinctada fucata. Because the treatment had no detectable deleterious effects on the growth and survival of the animals studied, the application of the method to the marine mineralized samples such as coccolithophores, reef-building corals and mollusks should promise the development not only in marine biology but also in aquaculture.

无论从基础还是应用的角度来看，生物矿化研究都是较为重要的研究课题之一。软体动物的壳包含大量有关其自身生长历史的信息，包括矿化和压力或疾病的持续状况。对这些信息的分析对于海洋生物学和水产养殖来说都是非常重要的因素。应用荧光物质来分析生长性能和硬组织形成在医学和牙科科学中很流行。鱼耳石也可以通过浸泡在一些荧光物质中来标记。本研究的目的是开发一种用荧光物质标记双壳类贝壳、珍珠等软体动物硬组织的方法，以获得硬组织形成的有效信息。为了确定标记巨牡蛎壳的最佳四环素 (TC) 和茜素络合酮 (ALC) 浓度和处理时间，测试了各种浓度和处理时间的组合。 TC在紫外光照射下牡蛎壳背腹面呈现清晰的黄色荧光线。最佳处理条件为200mg/l，处理12-24小时。 ALC以150-300mg/l处理12-24小时观察到橙色标记。 TC和ALC双重治疗取得了成功。在珠母贝中可以观察到贝壳和珍珠生长性能的相似性和差异。由于该处理对所研究动物的生长和生存没有可检测到的有害影响，因此将该方法应用于海洋矿化样品（如颗石藻、造礁珊瑚和软体动物）不仅有望促进海洋生物学的发展，而且也有望促进水产养殖业的发展。