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Studies on the method for searching endocrin disrupting chemicals and the regulation of accumulation of the chemicals in the body of animals

内分泌干扰物的寻找方法及其在动物体内蓄积调控的研究

基本信息

批准号：
13660334
负责人：
AOYAGI Yosuke
金额：
$ 2.18万
依托单位：
IBARAKI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

This study was conducted in an attempt to search endocrin disrupting chemicals, using male chickens or cultured hepatocytes of male chickens. The vitellogenin mRNA expression was selected as an index. Vitellogenin is the egg yolk protein expressed in the liver of female chickens. The chemicals with estrogen-like effect induce the vitellogenin expression in the liver of male chickens. Vittellogenin mRNA was detected using the rt-PCR methods.Using this method, we found the estrogen-like effect of an insecticide (Name of the chemical is not shown. ).Furthermore, we investigeted the pollution by endocrin disrupting chemicals of the water of Lke Kasumigaura, using male chickens. The vitellogenin mRNA expression could not be detected in the liver of male chickens administered the lake water.A primary culture of hepatocytes was applied to experimental models of liver function. In this study, cultured hepatocytes with non-serum medium were used to search endcrin disrupting chemicals. Vitellogenin mRNA expression was detected in cultured hepatocytes with or without estrogen. This noize could be eliminate within this project term.

本研究试图利用公鸡或培养的公鸡肝细胞寻找干扰内分泌的化学物质。以卵黄蛋白原mRNA的表达为指标。卵黄蛋白原是母鸡肝脏中表达的蛋黄蛋白。具有雌激素样作用的化学物质可诱导雄鸡肝脏中卵黄蛋白原的表达。用RT-PCR方法检测Vittellogenin的mRNA，用这种方法，我们发现了一种杀虫剂(化学物质的名称没有显示)的雌激素样作用。)。此外，我们还以雄鸡为实验对象，对鹿浦湖水中内分泌干扰物的污染情况进行了调查。在饮用湖水的公鸡肝脏中未检测到卵黄蛋白原基因的表达，将肝细胞原代培养应用于肝功能实验模型。本研究利用无血清培养的肝细胞寻找内分泌干扰物。在有或无雌激素的培养肝细胞中均检测到卵黄蛋白原基因的表达。这种噪音可以在这个项目期限内消除。