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Effects of Dietary Chitosan and Genistein on Calcium and Bone Metabolism in Ovariectomized Rats

膳食壳聚糖和染料木黄酮对去势大鼠钙和骨代谢的影响

基本信息

批准号：
13670072
负责人：
OTA Fukio
金额：
$ 1.73万
依托单位：
Waseda University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670072/
关键词：
Chitosan Genistein Ovariectomized rat Resistance walking Bone mineral density Urinary Ca excretion Serum 1,25(CH)_2D_3 Ca binding protein D 9K mRNA 投与開始時期血中1,25(OH)_2 小腸Ca結合タンパク質mRNA 小腸ビタミンD受容体mRNA P腸管吸収阻害卵巣摘出ラットレジスタンス運動カ

项目摘要

Effects of both chitosan, a cationic dietary fiber, and genistein, a soybean phytoestrogen were investigated on bone status and Ca metabolism in ovariectomized (OVX) rats as a model of postmenopausal women.1. Lowering effects of chitosan on the bone mineral density (BMD) and the mechanism by which it decreases BMD; Chitosan caused a significant reduction in BMD and stiffness in femurs and vertebrae of OVX rats fed a low Ca diet for 40 days, as compared to cellulose. There was no remarkable difference in intestinal Ca absorption between chitosan and cellulose, whereas chitosan significantly decreased P absorption. The bone loss in rats fed chitosan was accompanied with a marked increase in urinary Ca excretion and a decrease in serum Ca level, and with a significant increment in serum PIH and 25(CH)_2D_3. Chitosan also significantly increased vitamin D receptor and Ca binding protein D9K mRNAs of the duodenum. These results indicate that dietary chitosan possibly induces the decrease in BMD by enhancing urinary Ca excretion through depressed P absorption, which causes the lowering of serum inorganic P level resulting in the increase in circulating PIH and 1, 25(CH)_2D_3.2. Suppressive effects of genistein dosage and resistance walking on postmenopausal bone loss; Genistein dosage at 5mg/day for 4 weeks to OVX rats significantly lowered the postmenopausal decrease in BMD of femurs, but not of vertebrae. For complementing the preventive function of genistein to vertebrae loss, the effects of different moderate exercises were evaluated. Resistance exercise constituted of 1-min walking interspersed with 1-min rest with 100g weight on the back successively reduced the postmenopausal BMD decrease in vertebrae, but not in the case of ordinary walking. The combined treatment of genistein and resistance walking acted independently to the prevention of postmenopausal bone loss.

以绝经后妇女模型为对象，研究了壳聚糖（一种阳离子膳食纤维）和金雀异黄酮（一种大豆植物雌激素）对卵巢切除（OVX）大鼠骨状态和钙代谢的影响。 1．壳聚糖对骨矿物质密度（BMD）的降低作用及其降低BMD的机制；与纤维素相比，壳聚糖导致喂食低钙饮食 40 天的 OVX 大鼠的 BMD 以及股骨和椎骨硬度显着降低。壳聚糖和纤维素的肠道Ca吸收没有显着差异，而壳聚糖显着降低P吸收。饲喂壳聚糖的大鼠骨丢失伴随着尿Ca排泄显着增加和血清Ca水平降低，并且血清PIH和25(CH)_2D_3显着增加。壳聚糖还显着增加十二指肠的维生素 D 受体和 Ca 结合蛋白 D9K mRNA。这些结果表明，膳食壳聚糖可能通过抑制磷的吸收而增加尿钙排泄，从而导致血清无机磷水平降低，导致循环PIH和1, 25(CH)_2D_3.2增加，从而导致骨密度下降。金雀异黄素剂量和阻力行走对绝经后骨质流失的抑制作用；对 OVX 大鼠给予 5 毫克/天的金雀异黄素剂量，持续 4 周，可显着降低绝经后股骨 BMD 的下降，但不能降低椎骨的 BMD 下降。为了补充金雀异黄酮对椎骨丢失的预防作用，评估了不同适度运动的效果。步行1分钟，后背负重100克休息1分钟的抗阻运动，可相继减少绝经后椎骨骨密度的下降，但普通步行则不然。金雀异黄素和阻力行走的联合治疗对于预防绝经后骨质流失独立起作用。