Molecular mechanisms of inactivation process of L-type Ca2+ channel

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Calcium channel currents activate with membrane depolarization and inactivate overtime. The inactivating property has an important role in regulating intracellular Ca2+ concentration. Inactivation of the currents is modulated by at least tree factors : 1)membrane potential, 2) Ca2+, and 3) phosphorylation.In this study, we investigated molecular mechanisms of the inactivation process related to phosphorylation using expression system. We cloned CACNA1C from guinea pig heart. And we investigated the role of phosphorylation sites in CACNA1C with mutagenesis study using whole-cell patch clamp study.Our data indicate that 1) multiple phosphorylation sites in CACNA1are involved in the regulation of the channel activity, 2)other subunit regulates roles of each phosphorylation site in CACNA1C.We have cloned CACNA1C(AB092629) and KCNJ12 (AB074970).