Molecular pathology of tumor cell origin and the oncogenesis in renal angiomyolipoma

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The different types of the vascular component of the renal angiomyolipoma were classified histologically into muscular artery, small muscular artery, muscular vein, capillary and staghorn type / sinusoid type vessels.The histopathologic analysis of proliferative smooth muscle cells in concentric proliferative pattern revealed the invasive behavior of the proliferative smooth muscle cells to gathered proliferative arteries.We established the analysis system of the fragment length and the quantity of the PCDR product in two different-sized X-linked HUMARA gene in Japanese women.We sampled the different component of the renal angiomyolipoma selectively, utilizing the "LM 200 Olympus microdissection microscope", amplified the HUMARA gene, and analyzed them to determine the neoplastic tendency of the component cells.The smooth muscle cells of normal appearing vessels were polyclonal by HUMARA analysis, demonstrating their non-neoplastic nature.On the other hand, the proliferating smooth muscle cells showed disappearance or significant decrease of the amount of one peak of HUMARA gene amplification product, indicating their monoclonal nature.We are now preparing to investigate other proliferative lesions that have not been decided neoplastic or non-neoplastic in nature, as well as other components of the renal angiomyolipoma.