Molecular pathology of tumor cell origin and the oncogenesis in renal angiomyolipoma

肾错构瘤血管成分的不同类型在组织学上可分为肌性动脉、肌性小动脉、肌性静脉，毛细管和鹿角型/结果显示，在血管壁中，平滑肌细胞呈向心性增殖，并对血管壁中的血管进行了组织病理学分析，揭示了平滑肌细胞对血管壁的侵袭行为，建立了血管壁中平滑肌细胞向心性增殖的分析系统，本研究通过对日本女性两种不同大小的X-连锁HUMARA基因PCDR产物的长度和数量的测定，选择性地对肾血管平滑肌脂肪瘤的不同组成部分进行取样，利用“LM 200 Olympus显微解剖显微镜”扩增HUMARA基因，并对其进行分析，以确定组成细胞的肿瘤倾向。另一方面，增生的平滑肌细胞显示HUMARA基因扩增产物的一个峰消失或显著减少，表明它们是单克隆的。我们现在准备研究其他尚未确定肿瘤性或非肿瘤性的增生性病变，以及肾血管平滑肌脂肪瘤的其他成分。

The different types of the vascular component of the renal angiomyolipoma were classified histologically into muscular artery, small muscular artery, muscular vein, capillary and staghorn type / sinusoid type vessels.The histopathologic analysis of proliferative smooth muscle cells in concentric proliferative pattern revealed the invasive behavior of the proliferative smooth muscle cells to gathered proliferative arteries.We established the analysis system of the fragment length and the quantity of the PCDR product in two different-sized X-linked HUMARA gene in Japanese women.We sampled the different component of the renal angiomyolipoma selectively, utilizing the "LM 200 Olympus microdissection microscope", amplified the HUMARA gene, and analyzed them to determine the neoplastic tendency of the component cells.The smooth muscle cells of normal appearing vessels were polyclonal by HUMARA analysis, demonstrating their non-neoplastic nature.On the other hand, the proliferating smooth muscle cells showed disappearance or significant decrease of the amount of one peak of HUMARA gene amplification product, indicating their monoclonal nature.We are now preparing to investigate other proliferative lesions that have not been decided neoplastic or non-neoplastic in nature, as well as other components of the renal angiomyolipoma.