Distribution of ABO blood group H type 1-4 sugar chain antigens in human tissue from secretor or non-secretor individuals

1.用免疫组织化学方法明确了H型抗原1、H型2和H型3/4在人体组织中的分布。肺内分泌型支气管腺粘液细胞呈H型抗体染色，支气管壁顶端呈H型和H型3/4强阳性。然而，两种抗体均不能使非分泌型抗体染色。无论是分泌型还是非分泌型，H2型抗体均可使支气管表皮基底层细胞染色。分泌型和非分泌型肾远端曲管H2型和H型3/4染色。分泌型集合管主要H1型抗体染色，非分泌型集合管几乎不染色。此外，只有来自分泌者的集合管被H型3/4抗体染色。2.Northern印迹和原位杂交分析表明，FUT1和FUT2基因在mRNA水平表达。我们使用腺癌细胞系(MCAS、PC-3、CCK-81、CoCM-1、WiDR和SW837)和表皮样癌细胞系(HeLa和Hep-2)。除HeLa、Hep-2和PC-3外，FUT1在所有人癌细胞中均有表达，FUT 2在所有细胞中均有表达。结果表明，FUT和FUT在癌细胞中均有表达，而在表皮样癌细胞中仅有FUT1的表达。此外，从横结肠到直肠(从CCK-81到SW837，见上)的腺癌细胞系中，FUT1 mRNA的表达量增加。我们将已报道的岩藻糖基转移酶在不同癌症组织中的表达数据与我们的研究结果进行了比较，发现岩藻糖基转移酶在癌组织中的异常表达受到一定水平的mRNA的调控。

1.The distribution of H type1, H type2, and H type 3/4 antigens in human tissue were clarified by use of immunohistochemical techniques. In lungs, mucous cells of a bronchus gland of secretor type was stained by H type1 antibody, and the tip of the bronchus mucous membrane surface was by H type1 and H type3/4 strongly. However, neither of antibodies stained that of non-secretor. The basal cells of bronchus epidermis were stained by H type2 antibody regardless of secretor or non-secretor. The distal convoluted tubule of kidney derived from secretor or non-secretor was stained by H type2 and H type3/4. The collecting duct derived from secretor was stained by H type1 antibody mainly, and it was not stained by that from non-secretor very much. In addition, only the collecting duct from secretor was stained by H type3/4 antibody.2.With Northern blot and in situ hybridization analysis, we demonstrated expressions of FUT1 and FUT2 gene at the level of mRNA. We used adenocarcinoma cell lines (MCAS,PC-3,CCK-81,CoCM-1,WiDr, and SW837) and epidermoid cancer cell lines (HeLa and HEp-2). FUT1 was expressed in all of human carcinoma cell lines, and FUT 2 was expressed in all of cells except HeLa,HEp-2, and PC-3. As a result, the tendency that both of FUTs were expressed in the carcinoma cells, and only FUT1 mRNA was expressed in the epidermoid cancer cells. In addition, the quantity of expressions of FUT1 mRNA increased in adenocarcinoma cell lines from a transverse colon to the rectum (from CCK-81 to SW837, see above). We compared the data have been reported about expression of the fucosyltransferase in various cancer tissue with the result of our research, and found that aberrant expressions of fucosyltransferase in the cancer tissue were regulated by a level of mRNA.