Therapeutic trial of prion infected mice by continuous infusion of anti-prion agent to brain

1)我们建立了朊病毒感染的细胞系，以评价抗朊病毒药物的体外作用; 2）筛选了可能具有抗朊病毒作用的化学药物和现有药物; 3）在我们测试的药物中，戊聚糖多硫酸酯（PPS）显示出最大的抗朊病毒作用; 4）为了获得增加脑递送率，5）Morphorino在体外不能抑制PrPc的产生。6）我们建立了用PPS和奎纳克林治疗朊病毒病的临床方案。

1) We established prion-infected cell line to evaluate in-vitro effect of anti-prion agents.2) We screened chemical agents and already existing medicines which may have possible anti-prion effect.3) Pentosan Polysulfate (PPS) showed maximum anti-prion effect among the agents we tested.4) To acquirer increased ratio of brain delivery, we are try to modifying PPS by chemical reactions.5) Morphorino failed to reduce PrPc production in vitro.6) We established the clinical protocol of patients with Prion disease using PPS and Quinacrine.