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Mechanism of coronary artery anomalies and myocardial growth disturbance in bis-diamine treated rat using whole embryo culture technique

全胚胎培养技术双二胺治疗大鼠冠状动脉异常和心肌生长障碍的机制

基本信息

批准号：
13670797
负责人：
FUJINO Hidetoshi
金额：
$ 1.98万
依托单位：
Shiga University of Medical Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

1. The embryos were removed from pregnant Wistar rats on 10.5 day of gestation and whole embryo culture was performed to observe the development of the pericardium serially. The developing pericardium was first noted 12 hours after beginning of culture from the transverse septum and started to cover over the embryonic heart. At 36 hours after beginning of culture, the heart was completely covered with the pericardium and, subsequently, the pericardium was approaching to the surface of the heart as the development was advanced.2. Bis-diamine was administered to the pregnant rats on 10.5 day of gestation and the mother rats were sacrificed three hours after administration for whole embryo culture. The development of the pericardium was delayed in the cultured embryo treated with bis-diamine and the whole heart was not covered with the pericardium even 36 hours after beginning of culture. The defects of the pericardium were found in some cultured embryos. The pericardium developed later, but was not close to the surface of the heart.3. Bis-diamine was given to the mother rats on 10.5 day of gestation and the embryos were removed from 14.5 to 17.5 day of gestation. BrdU was administered to the rats intraperitoneally one hour before the rats were sacrificed. The control embryos without treatment of bis-diamine showed that the uptake of BrdU to the ventricular wall muscle was increased on 15.5 day of gestation. In the embryos treated with bis-diamine, the ventricular wall was quite thin and the uptake of BrdU was diminished.These results suggested that bis-diamine delayed the development of the pericardium and prevent from the contact of the pericardium to the ventricular surface and, subsequently, the establishment of the coronary circulation was retarded. The thin ventricular wall observed in the embryos treated with bis-dimaine may be caused by the insufficient blood flow to the developing myocardium.

1.孕10.5天取孕鼠胚胎，进行全胚胎培养，动态观察心包的发育。从横隔开始培养12小时后，首先观察到发育中的心包，并开始覆盖在胚胎心脏上。在培养开始后36小时，心脏完全被心包覆盖，随后，随着发育的进行，心包接近心脏的表面。在妊娠第10.5天对妊娠大鼠给予双二胺，并在给药后3小时处死母鼠进行全胚胎培养。在用双二胺处理的培养胚胎中，心包的发育被延迟，并且即使在培养开始后36小时，整个心脏也没有被心包覆盖。部分培养胚胎出现心包缺损。心包发育较晚，但不紧贴心脏表面.孕10.5天给予双二胺，孕14.5 ~ 17.5天取出胚胎。在处死大鼠前1小时腹腔注射BrdU。未用双二胺处理的对照胚胎显示，在妊娠15.5天，心室壁肌肉对BrdU的摄取增加。双二胺处理的胚胎心室壁较薄，BrdU摄取减少，表明双二胺延缓了心包的发育，阻止了心包与心室表面的接触，从而延缓了冠状循环的建立。在用双迪马因处理的胚胎中观察到的心室壁较薄可能是由于发育中心肌的血流不足引起的。