The Research on Extension of the Viable Period in Islet Transplantation

点击翻译按钮获取中文摘要

We performed homogeneous transplantation using rat islet to find a method for extension of the viable period in islet transplantation. We isolated and purified rat islet from rat pancreas. We can get almost same number of viable islets from rat pancreas and we can make diabetic rats from injecting streptozotocine to rats. First, we tried to make the microcapsule of islet. But, we failed to do it. So, we directly injected islets to portal vein. The data that we got from the method were not stabilized. The period whose blood sugar level was stable was extremely short. We were not able to obtain the result stabilized even if it changed various methods of medication course. It did not clarify about participation of apoptosis in islet transplantation. We performed expression analysis of the Reg protein that is considered to extend a viable period of islets. We inserted cDNA of the Reg protein that was obtained using RT-PCR to expression vectors. The expression efficiency of Reg protein was quite bad. We were not able to get a required quantity of the protein for analysis.