The Research on Extension of the Viable Period in Islet Transplantation

胰岛移植存活期延长的研究

• 批准号: 13671318
• 负责人: HONMA Toshio
• 金额: $ 1.34万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671318/
• 关键词: diabetes mellitus; islet transplantation; artificial pancreas

We performed homogeneous transplantation using rat islet to find a method for extension of the viable period in islet transplantation. We isolated and purified rat islet from rat pancreas. We can get almost same number of viable islets from rat pancreas and we can make diabetic rats from injecting streptozotocine to rats. First, we tried to make the microcapsule of islet. But, we failed to do it. So, we directly injected islets to portal vein. The data that we got from the method were not stabilized. The period whose blood sugar level was stable was extremely short. We were not able to obtain the result stabilized even if it changed various methods of medication course. It did not clarify about participation of apoptosis in islet transplantation. We performed expression analysis of the Reg protein that is considered to extend a viable period of islets. We inserted cDNA of the Reg protein that was obtained using RT-PCR to expression vectors. The expression efficiency of Reg protein was quite bad. We were not able to get a required quantity of the protein for analysis.

本实验采用大鼠胰岛进行同种异体移植，旨在寻找一种延长胰岛移植存活期的方法。从大鼠胰腺中分离纯化大鼠胰岛。从大鼠胰腺中可以获得几乎相同数量的活胰岛，并且通过注射链脲佐菌素可以建立糖尿病大鼠模型。首先，我们尝试了胰岛的微胶囊化。但是，我们没有成功，所以我们直接将胰岛注射到门静脉。我们从该方法中得到的数据不稳定。血糖水平稳定的时间非常短。即使改变了各种给药方法，也无法获得稳定的结果。细胞凋亡在胰岛移植中的作用尚不明确。我们进行了Reg蛋白的表达分析，认为其延长了胰岛的存活期。我们将使用RT-PCR获得的Reg蛋白的cDNA插入表达载体。Reg蛋白的表达效率很差。我们无法获得所需数量的蛋白质进行分析。

项目成果

K Hirata: "Clinical and Basic Aspects in Emergent Abdominal Diseases of the liver, Biliary System, and Pancreas"Journal of Abdominal Emergency Medicine. 23. 13-26 (2003)

K Hirata：“肝脏、胆道系统和胰腺的紧急腹部疾病的临床和基本方面”腹部急诊医学杂志。

水口 徹, 平田公一ら: "組織再生とES細胞-上皮細胞移植の将来-"日本腹部救急医学会雑誌. 22. 1015-1021 (2002)

Toru Mizuguchi、Koichi Hirata 等：“组织再生和 ES 细胞 - 上皮细胞移植的未来”日本腹部急救医学会杂志 22. 1015-1021 (2002)。

平田公一, 浦 英樹ら: "肝,胆,膵の救急疾患の診療と研究;EBMから分子生物学まで"日本腹部救急医学会雑誌. 23. 13-26 (2003)

Koichi Hirata、Hideki Ura 等：“肝脏、胆汁和胰腺紧急疾病的医疗治疗和研究；从 EBM 到分子生物学”日本腹部急救医学会杂志 23. 13-26 (2003)。

T Mizuguchi: "Regenerative Medicine and Embryonic Stem Cells -The Future of Epithelium Transplantation"Journal of Abdominal Emergency Medicine. 22. 1015-1021 (2002)

T Mizuguchi：“再生医学和胚胎干细胞-上皮移植的未来”腹部急诊医学杂志。