Mechanism of myocacardial preconditioning against ischemia reperfusion injury and effective means of improving function of heart transplants

心肌预处理抗缺血再灌注损伤的机制及改善心脏移植功能的有效手段

• 批准号: 13671389
• 负责人: ISHIGURO Shingo
• 金额: $ 2.24万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671389/
• 关键词: intra-cellular calcium overload; ischemia reperfusion injury; preconditioning; myocardial protection; heart preservation; 臓器保存法; 心保存液

Cardiac harvest teams are usually committed immediately transfer the explanted donor heart into its cold storage solution. We tested the opposite hypothesis that brief prestorage episode of ischemic preconditioning could be protected. This study was designed to study the effect of ischemic preconditioning in an isolated heart model of cold storage, simulating the situation of cardiac of cardiac allograft. Isolated isovolumic buffer-perfused rat hearts in normothermic Klebs-Henseleitsolution were arrested with and storage with cold Klebs-Henseleit solution at 4℃ for 4hours before a 1-hour reperfusion. Control hearts were arrested with and stored in K-H solution. Preconditioning group hearts were preconditioned by two 5 minutes episodes of global ischemia, each separated by 5 minutes of reperfusion before arrested with and stared in K-H solution. On the basis of comparisons of postischemic left ventricular contractility and diastolic function, and heart rate, values indicative of improved protection were obtained by ischemic preconditioning These data suggest that cardioprotection by ischemic preconditioning might effectively contribute to improve donor heart preservation during cardiac transplantation.

心脏采集团队通常会立即将经过冷冻的供体心脏转移到其冷藏溶液中。我们验证了相反的假设，即短暂的预缺血处理可以保护。本研究拟在离体心脏冷保存模型上，模拟同种异体心脏移植的情况，研究缺血预处理对心脏冷保存的影响。离体等容缓冲液灌流的大鼠心脏，在再灌注1小时前，用冷的Klebs-Henseleit液停搏并在4℃保存4 h。对照组心脏用K-H溶液停搏并保存。预处理组先进行2次5 min的全脑缺血预处理，再灌注5 min，然后用K-H液停搏并停跳。在比较缺血后左心室收缩和舒张功能以及心率的基础上，获得了缺血预处理改善保护的值。这些数据表明，缺血预处理的心脏保护可能有效地有助于改善心脏移植中供体心脏的保存。

项目成果

丸本明彬: "Isoprotetenolによる一過性内向き電流誘発不整脈モデルを用いた医薬品の催不整脈作用の検討"米子医学雑誌. 54・1. 33-47 (2003)

Akiaki Marumoto：“使用异丙醇的瞬时内向电流诱发心律失常模型检查药物的致心律失常作用”米子医学杂志 54・1（2003）。