Mechanism of myocacardial preconditioning against ischemia reperfusion injury and effective means of improving function of heart transplants

心脏采集小组通常承诺立即将移植的供体心脏转移到冷藏溶液中。我们测试了相反的假设，即短暂的缺血预处理前储存事件可以得到保护。本研究旨在模拟同种异体心脏移植后的心脏状况，研究缺血预处理对离体心脏冷藏模型的影响。在常温Klebs-Henseleit溶液中分离的等体积缓冲灌注的大鼠心脏，用低温Klebs-Henseleit溶液在4℃下捕集并保存4小时，再灌注1小时。对照心脏用K-H溶液捕获并保存。预处理组心脏进行两次5分钟的全脑缺血预处理，每次再灌注间隔5分钟，然后用K-H溶液停搏和凝视。通过对缺血后左心室收缩力、舒张功能和心率的比较，得出了缺血预处理可改善心脏保护的数值。这些数据表明，缺血预处理可有效改善心脏移植过程中供体心脏的保存。

Cardiac harvest teams are usually committed immediately transfer the explanted donor heart into its cold storage solution. We tested the opposite hypothesis that brief prestorage episode of ischemic preconditioning could be protected. This study was designed to study the effect of ischemic preconditioning in an isolated heart model of cold storage, simulating the situation of cardiac of cardiac allograft. Isolated isovolumic buffer-perfused rat hearts in normothermic Klebs-Henseleitsolution were arrested with and storage with cold Klebs-Henseleit solution at 4℃ for 4hours before a 1-hour reperfusion. Control hearts were arrested with and stored in K-H solution. Preconditioning group hearts were preconditioned by two 5 minutes episodes of global ischemia, each separated by 5 minutes of reperfusion before arrested with and stared in K-H solution. On the basis of comparisons of postischemic left ventricular contractility and diastolic function, and heart rate, values indicative of improved protection were obtained by ischemic preconditioning These data suggest that cardioprotection by ischemic preconditioning might effectively contribute to improve donor heart preservation during cardiac transplantation.