Analysis of the antigen presentation machinery in malignant glioma cells

抗原加工相关转运蛋白（TAP）是肽转运和抗原提呈所必需的。本研究探讨了TAP 1在恶性胶质瘤细胞中的表达及IFN-γ、IFN-β对TAP 1表达的影响。我们还研究了TAP 1启动子的多态性。我们分析了八个恶性胶质瘤细胞中TAP 1启动子的序列。8个恶性胶质瘤细胞中有6个出现单核苷酸多态性。该多态性是TAP 1翻译起始点上游446 bp处的G-T取代。TAP 1在所有恶性胶质瘤细胞中均呈低表达。IFN-γ和IFN-β可促进TAP 1蛋白和mRNA的表达。TAP 1启动子的多态性对IFN-γ和IFN-β诱导TAP 1表达无功能性影响。

Transporter associated with antigen processing (TAP) is necessary for peptide transport and antigen presentation. We investigated a expression of TAP1 in malignant glioma cells and the effect of IFN-γ, IFN-β on the expression of TAP1. We also investigated the polymorphism of TAP1 promoter. We analyzed the sequence of TAP1 promoter in eight malignant glioma cells. Single nucleotide polymorphism occurred in six of eight malignant glioma cells. This polymorphism is a G-T substitution 446 bp upstream of the translation start of TAP1. The expression of TAP1 in all malignant glioma cells was very low. IFN-γ and IFN-β increased the protein and mRNA expression of TAP1. The polymorphism of TAP1 promoter did not have functional effects on the induction of TAP1 expression by IFN-γ and IFN-β.