Analysis of the antigen presentation machinery in malignant glioma cells

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Transporter associated with antigen processing (TAP) is necessary for peptide transport and antigen presentation. We investigated a expression of TAP1 in malignant glioma cells and the effect of IFN-γ, IFN-β on the expression of TAP1. We also investigated the polymorphism of TAP1 promoter. We analyzed the sequence of TAP1 promoter in eight malignant glioma cells. Single nucleotide polymorphism occurred in six of eight malignant glioma cells. This polymorphism is a G-T substitution 446 bp upstream of the translation start of TAP1. The expression of TAP1 in all malignant glioma cells was very low. IFN-γ and IFN-β increased the protein and mRNA expression of TAP1. The polymorphism of TAP1 promoter did not have functional effects on the induction of TAP1 expression by IFN-γ and IFN-β.