Gene expression and apoptosis in the slow neuronal death.

首先，我们研究了热休克/应激蛋白70(HSP70)在单侧大脑中动脉(MCA)凝固模型和单侧线栓模型中表达和蛋白质合成的差异。在MCA凝血模型中，缺血区(包括同侧皮质和尾状核)HSP70mRNA和HSP70蛋白表达及蛋白质合成减少。然而，在MCA区域以外的区域，包括同侧海马、丘脑和黑质下部，没有观察到这些现象。这些结果在实验大鼠中是一致的。然而，在铝内线模型中，在MCA区域以外的区域，包括同侧海马、丘脑和黑质，以及缺血区，包括同侧皮质和尾状核，都观察到HSP70 mRNA和HSP70蛋白的诱导和蛋白质合成的损害。这些结果在实验大鼠中并不一致。这些不同的结果可能是由于铝内穿线模型中ICA闭塞造成的广泛损害。因此，我们建议将这一模型称为ICA闭塞模型，而不是单纯的MCA闭塞模型。通过这两种模型，我们正在确定使用地高辛标记的RNA探针进行非放射性Northern印迹的最佳条件。

First of all, we studied the differences between heat-shock/stress protein70 (hsp70) gene expression and protein synthesis in the unilateral middle cerebral artery (MCA) coagulation (Tamura's) model and unilateral intraluminal threading model. In the MCA coagulation model, expression of hsp70 mRNA and hsp70 protein and decreased protein synthesis were detected in the ischemic areas, including the ipsilateral cortex and caudate. However these phenomena were not observed in the areas outside the MCA territory, including the ipsilateral hippocampus, thalamus and substanda nigra. These results were consistent among the experimental rats. In the intraluminai threading model, however, induction of both hsp70 mRNA and hsp70 protein and impairment of protein synthesis were noted in the areas outside the MCA territory, including the ipsilaterai hippocampus, thalamus and substantia nigra, as well as in the ischemic areas, including the ipsilateral cortex and caudate. These results were not consistent among the experimental rats. These different results might be due to widespread damage resulting from ICA occlusion in the intraluminai threading model. Accordingly, we propose that this model should be called an ICA occlusion model, rather than a pure MCA occlusion model.Concerning gene expression and apoptosis in the slow neuronal death following ischemia by these two models, we are confirming the optimal condition for non-radioactive northern blot using digoxigenin-labeled RNA probe.