The breakdown and recovery of blood-nerve barrier

血神经屏障的破坏与恢复

• 批准号: 13671501
• 负责人: NAGANO Akira
• 金额: $ 1.15万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671501/
• 关键词: blood-nerve barrier; endothelial barrier antigen; rat endothelial cell antigen-1; quantitative analysis; evans blue albumin; peripheral nervous system; sciatic nerve; crush injury; 神経再生; 免疫組織学的定量

The purpose of this study is to reveal whether the application of immunohistochemical examinations to the peripheral nervous system (PNS) can be a reliable method for the quantitative analysis of the blood-nerve barrier (BNB) and the relationship between restoration of BNB and nerve regeneration. Sciatic nerves in rats were examined after nerve crush. Immunohistochemical staining with anti-rat endothelial cell antigen-1 (anti-RECA-1) that recognizes endothelial cells and anti-endothelial barrier antigen (anti-EBA) for the detection of barrier-type endothelial cells were used. Anti-neurofilament for staining axons was also performed. A quantitative analysis of the BNB was assessed using the ratio of EBA positive cells and RECA-1 positive cells. The ratio of EBA/RECA-1 decreased significantly three days postoperatively and reached its lowest level at day seven in the segment 5mm proximal and the entire distal stump. The ratio gradually recovered from the proximal and the regeneration of axons started a week earlier than BNB. The ratio of EBA/RECA-1 applied to the PNS can be a reliable method for the quantitative analysis of BNB. In crush injuries, the breakdown of BNB occurred simultaneously in the segment 5mm proximal and the entire distal stump ; restoration began from the proximal to distal and followed a week later to nerve regeneration.

本研究旨在探讨外周神经系统（PNS）免疫组化检测能否作为定量分析血-神经屏障（BNB）及其修复与神经再生关系的可靠方法。大鼠坐骨神经夹伤后进行检查。使用识别内皮细胞的抗大鼠内皮细胞抗原-1（anti-RECA-1）和用于检测屏障型内皮细胞的抗内皮屏障抗原（anti-EBA）进行免疫组织化学染色。还进行了用于染色轴突的抗神经丝。使用EBA阳性细胞和RECA-I阳性细胞的比率评估BNB的定量分析。EBA/RECA-1的比值在术后3天显著下降，并在术后7天在近端5 mm和整个远端残端达到最低水平。从近端开始逐渐恢复，轴突再生开始时间比BNB早一周。EBA/RECA-1比值用于PNS的定量分析是一种可靠的方法。在挤压伤中，BNB的崩溃同时发生在近端5 mm和整个远端残端的节段;从近端到远端开始恢复，一周后神经再生。

项目成果

大村久美子 佐野倫生 大村威夫 長谷川智彦 長野 昭 大林雅春: "血液-神経関門の機能動態"日本整形外科学会雑誌. 76巻8号. S955 (2002)

Kumiko Omura、Michio Sano、Takeo Omura、Tomohiko Hasekawa、Akira Nagano、Masaharu Obayashi：“血神经屏障的功能动力学”日本骨科学会杂志，第 76 卷，第 8 期（2002 年）。

Kumiko Omura, Michio Sano, Akira Nagano, et al: "Blood-nerve barrier (Japanese)"NIPPON SEIKEIGEKAGAKKAI ZASSHI. S955. 76-78 (2002)

Kumiko Omura、Michio Sano、Akira Nagano 等：“血神经屏障（日语）”NIPPON SEIKEIGEKAGAKKAI ZASSSHI。

大村久美子, 佐野倫生, 長野 昭 ほか: "血液-神経関門の機能動態"日本整形外科学会雑誌. 76巻8号. S955 (2002)

Kumiko Omura、Michio Sano、Akira Nagano 等人：“血神经屏障的功能动力学”，日本骨科学会杂志，第 76 卷，第 8 期（2002 年）。