Research for Residual bladder function using molecular biology and Suitable period of surgical treatment of Benign Prostatic Hyperplasia

良性前列腺增生症残余膀胱功能的分子生物学研究及手术治疗适宜期

• 批准号: 13671681
• 负责人: OHNISHI Norio
• 金额: $ 2.3万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671681/
• 关键词: Benign Prostatic Hyperplasia(BPH); Residual bladder function; Suitable period of surgical treatment; ミトコンドリア

This paper reports the evaluation of the residual bladder function in patients with bladder outlet obstruction(BOO) such as those with benign prostatic hyperplasia(BPH), and determination of the appropriate timing for a surgical procedure. The study mainly researched factors contributing to preserve the bladder function, the use of culturing protain on bladder smooth muscle cells, and their structural changes.First of all, we examined factors affecting the residual bladder function in patients with animal BOO model. From these experiments, we found that the influence by chronic bladder ischemia that was derived from BOO, and following bladder blood flow reperfusion injury are important factors. In addition, we found that free radicals and active oxygen affect the bladder function. Accordingly, we found that dosing free radical scavengers and vitamin E provide relief for the bladder dysfunction. As for the bladder ischemia-reperfusion animal model, we noticed that neutrophil elastase in … More hibitors have a protective effect on bladder smooth muscle, and various factors related to the residual bladder function. As to the relationship with the bladder blood flow, we also examined urination load timing(diuresis). The results showed that even diuresis before BOO, by stopping diuresis after BOO, protection and preservation effects of the bladder smooth muscle decreased and disappear, and it was found that diuresis affects the residual bladder function. Next, the study of cultured bladder smooth muscle cells in rats after following BOO indicated that the bladder hypertrophy of bladder was derived from the smooth muscle layer, and it was possible that b-FGF is one of growth factor. Further, after examining the structural change of the bladder smooth muscle cells in rats using a phenotype concept, the ratio of non-contractile phenotype was increased in the bladder smooth muscle cells after BOO. At the same time, using a molecular biological method, we measured protein markers that are related to bladder contraction. Moreover, the result showed that caldesmon is the most useful marker for grasping a morbid state. We tested this method on human bladder smooth muscle cells and found that levels of non-contractile phenotype was increased in bladder after BOO and bladder dysfunction, and the change of these can be parameters to determine the suitable period of surgical procedures for the BPH. Less

本文报告了对膀胱出口梗阻（BOO）患者（如良性前列腺增生（BPH）患者）的残余膀胱功能进行评估，并确定适当的手术时机。本研究主要研究膀胱功能保留的影响因素、培养蛋白在膀胱平滑肌细胞上的应用及其结构变化。从这些实验中，我们发现，由BOO引起的慢性膀胱缺血的影响，以及随后的膀胱血流再灌注损伤是重要的因素。此外，我们还发现自由基和活性氧也会影响膀胱功能。因此，我们发现给药自由基清除剂和维生素E可以缓解膀胱功能障碍。在膀胱缺血再灌注动物模型中，我们注意到， ...更多信息 抑制剂对膀胱平滑肌和与残余膀胱功能相关的各种因素具有保护作用。至于与膀胱血流量的关系，我们还检查了排尿负荷时间（利尿）。结果表明，即使在BOO前利尿，BOO后停止利尿，膀胱平滑肌的保护和保存效果下降和消失，发现利尿影响残余膀胱功能。其次，对BOO后培养的大鼠膀胱平滑肌细胞的研究表明，膀胱的膀胱肥大来源于平滑肌层，b-FGF可能是生长因子之一。此外，在使用表型概念检查大鼠膀胱平滑肌细胞的结构变化后，BOO后膀胱平滑肌细胞中非收缩性表型的比例增加。与此同时，使用分子生物学方法，我们测量了与膀胱收缩相关的蛋白质标记物。此外，结果表明，钙调素是最有用的标志，掌握一种病态。我们在人膀胱平滑肌细胞上测试了这种方法，发现BOO和膀胱功能障碍后膀胱中的非收缩表型水平增加，这些变化可以作为确定BPH手术操作的合适时期的参数。少

项目成果

Hanai T, Matsumoto S, Yamamoto K, Munakata H, Kurita T: "Examination of growth promotion factors for smooth muscle cells of hypertrophied bladders in rats following Partial outlet obstruction"Acta Medica Kinki University. 28(2). 47-54 (2003)

Hanai T、Matsumoto S、Yamamoto K、Munakata H、Kurita T：“部分出口梗阻后大鼠肥大膀胱平滑肌细胞生长促进因子的检查”近畿大学医学学报。

Ohnishi N, Yoshioka N, Onoe M., Hanai T, Matsumoto S, Miyatake R, Sugiyama T, Kurita T, Kajimoto N: "Protective effect of vitamin E on the response of the rat bladder dysfunction due to ischemic damage."Jin Hinyoukika Ybou Igaku(Japanese). 10(1). 62-63 (2

Ohnishi N、Yoshioka N、Onoe M.、Hanai T、Matsumoto S、Miyatake R、Sugiyama T、Kurita T、Kajimoto N：“维生素 E 对缺血性损伤引起的大鼠膀胱功能障碍反应的保护作用。”Jin Hinyoukika

大西規夫, 吉岡伸浩, 尾上正浩, 他: "酸化的ストレスによる虚血性膀胱機能障害に対する高容量ビタミンE投与の予防効果について"日本腎泌尿器科予防医学会誌. 10(1). 62-63 (2002)

Norio Onishi、Nobuhiro Yoshioka、Masahiro Onoe 等人：“高剂量维生素 E 对氧化应激引起的缺血性膀胱功能障碍的预防作用”，日本预防肾病和泌尿学会杂志 10(1)。 63（2002））

Hanai T, Ma FH, Matsumoto S, Park YC, Kurita T: "Partial outlet obstruction of the rat bladder induces a stimulatory response on proliferation of the bladder smooth muscle cells."International Urology Nephrology. 34(1). 37-42 (2002)

Hanai T、Ma FH、Matsumoto S、Park YC、Kurita T：“大鼠膀胱的部分出口阻塞会诱导膀胱平滑肌细胞增殖的刺激反应。”国际泌尿肾病学。

Matsumoto S, Hanai T, Ohnishi N, Yamamoto K, Kurita T: "Bladder smooth muscle cell phenotypic changes and implication of expression of contractile proteins(especially caldesmon) in rats after partial outlet obstruction."Int J Urol. 10(6). 339-345 (2003)

Matsumoto S、Hanai T、Ohnishi N、Yamamoto K、Kurita T：“部分出口梗阻后大鼠膀胱平滑肌细胞表型变化和收缩蛋白（尤其是钙结合蛋白）表达的意义。”Int J Urol。