EVALUATION OF OOCYTES QUALITY THOROUGH ANALYSIS OF APOPTOTIC SIGNAL TRANSDUCTION IN FOLLICLES.

通过分析卵泡中的凋亡信号转导来评估卵母细胞质量。

• 批准号: 13671692
• 负责人: NAKAHARA Kenji
• 金额: $ 2.24万
• 依托单位: YAMAGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671692/
• 关键词: APOPTOSIS; OVARY; ATRESIA OF FOLLICLE; GRANULOSA CELL; INTRACELLULAR SIGNAL TRANSDUCTION

Fas-Fas ligand which is a major system of signal transduction of apoptosis was used for elucidation of apoptotic system in ovaries from preovulatory female mice (B6C3 strain). Fas antigen is on the surface of granulosa cell membrane. Intracellular signal transduction starts after Fas ligand combines to Fas antigen. Fas antigen was not found on the surface of oocyte even in the atretic follicle of mouse. Otherwhile Fas antigen and Fas ligand was found on the surface of granulosa cells. In situ hybridization showed that apoptotic granulosa cells producted Fas antigen and not Fas ligand. Fas ligands was producted in oocyte. Therefore oocytes hold the key to follicle development or atretic follicles. Protein C kinase mediates intracellular signal transduction of granulosa cells. Other signal transduction was suggested while blockage of intracellular transduction via protein C kinase was no use in follicle development. There was p38 protein which contains 4 kinds of isoforms (α,β,γ and δ). In those groups, p38α stimulates follicular apoptosis but p38β does not. Further investigation is necessary for detection of all of intracellular signal transduction for apoptosis in atretic follicles.

Fas-Fas配体是细胞凋亡的主要信号转导系统，用来研究排卵前期雌性小鼠(B6C3株)卵巢中的凋亡系统。Fas抗原位于颗粒细胞膜表面。细胞内信号转导是在Fas配体与Fas抗原结合后开始的。小鼠卵母细胞表面甚至闭锁卵泡内均未检测到Fas抗原。颗粒细胞表面有Fas抗原和Fas配体。原位杂交显示，凋亡的颗粒细胞产生Fas抗原，而不产生Fas配体。Fas配体在卵母细胞中产生。因此，卵母细胞是卵泡发育或闭锁卵泡的关键。蛋白C激酶介导颗粒细胞的细胞内信号转导。有人提出了其他信号转导的建议，而通过蛋白C激酶阻断细胞内转导在卵泡发育中没有作用。P38蛋白含有4种亚型(α，β，γ和δ)。在这些组中，p38α刺激卵泡凋亡，而p38β不能。闭锁卵泡的细胞内信号转导与细胞凋亡的关系有待进一步研究。

项目成果

Ohta N et al.: "Soluble CD44 in human ovarian follicular fluid"J Assist Reprod Genet. 18. 23-27 (2001)

Ohta N 等人：“人卵巢卵泡液中的可溶性 CD44”J Assist Reprod Genet。

Ohta N. et al: "Soluble CD44 in human ovarian follicular fluid."J Assist Reprod Genet. 18. 21-25 (2001)

Ohta N. 等人：“人卵巢卵泡液中的可溶性 CD44。”J Assist Reprod Genet。

Saito H.et al.: "Endometriosis and Oocyte Quality"Gynecol Obstet Invest. 53-suppl1. 46-51 (2002)

Saito H.等人：“子宫内膜异位症和卵母细胞质量”Gynecol Obstet Invest。

Kaneko T et al.: "Alteration of Glutathione Reductase Expression in the Female Reproductive Organs During the Estrous Cycle"Biol Reprod. 65. 1410-1416 (2001)

Kaneko T 等人：“发情周期期间女性生殖器官中谷胱甘肽还原酶表达的改变”Biol Reprod。

Saito H. et al: "Endometriosis and oocyte quality."Gynecol Obstet Invest. 53. 46-51 (2002)

Saito H. 等人：“子宫内膜异位症和卵母细胞质量。”Gynecol Obstet Invest。